HLA-B27 Antigen

Test Code

LAB869

Alias/See Also

528, HLA-B27 Ag, LAB27, HLA27, Human Leukocyte Antigen B27, HLAB27 AG, B27

CPT Codes
86812

Preferred Specimen

5 ml Whole blood in Green Na Heparin Tube

Minimum Volume

Minimum: 1 mL

Other Acceptable Specimens

ACD solution A (yellow-top)
ACD solution B (yellow-top)
EDTA (lavender-top)

Transport Container

Sodium heparin (green-top) (preferred)
             ACD solution A (yellow-top)
             ACD solution B (yellow-top)
             EDTA (lavender-top)

Transport Temperature

Ship room temperature

Specimen Stability

Room temperature: 7 Days
             Refrigerated: 5 Days
             Frozen: Unacceptable

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Received frozen; Hemolysis; Lithium heparin (green-top) tube; Clotted

Methodology
Flow Cytometry

Setup Schedule

SETUP DAY/TIME***
Tue-Sun P
*** P=12pm-6pm

Report Available

REPORT DAY/TIME***
Wed-Mon N
***P=12pm-6pm

Clinical Significance

HLA-B27 is found in 90% of patients with ankylosing spondylitis
and 80% in Reiter's disease. Ankylosing spondylitis affects 1 in
1000 Caucasians. Ankylosing spondylitis is 10 times more common
among individuals with HLA-B27 compared to individuals without
this antigen.

Additional Information
NCD-ABN HLA Typing

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.