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Respiratory Virus PCR Panel I
Test CodeLAB6249
Alias/See Also
RVPCR
91246
91246
CPT Codes
87632
Includes
Influenza A & B , Parainfluenza 1 – 4, Adenovirus,RSV
Preferred Specimen
3 mL Throat swab or Nasopharyngeal swab.
Minimum Volume
0.35 mL
Other Acceptable Specimens
0.85 mL Sputum or Bronchial lavage/wash or Nasopharyngeal aspirate/wash or Tracheal lavage/wash
Instructions
Respiratory Samples in M4 media:
Use sterile vials containing 3 mL of sterile M4 media. If using swabs, use only sterile Dacron or Rayon swabs.
Do not use calcium alginate swabs, as they may contain substances that inhibit PCR testing. Break applicator stick off near the tip to permit tightening of the cap. Sputum: Collect in sputim collection kit or a sterile, plastic container with a leak-proof cap. Bronchial Lavage/wash: Collect in a sterile container with a leak-proof cap. Respiratory Samples in M4 media: Use sterile vials containing 3 mL of sterile M4 media. If using swabs, use only sterile Dacron or Rayon swabs. Do not use calcium alginate swabs, as they may contain substances that inhibit PCR testing. Break applicator stick off near the tip to permit tightening of the cap. Sputum: Collect in sputim collection kit or a sterile, plastic container with a leak-proof cap. Bronchial Lavage/wash: Collect in a sterile container with aleak-proof cap. Nasopharyngeal aspirate/wash: Collect in a sterile container with a leak-proof cap. Tracheallavage/wash: Collect in a sterile container with a leak-proof cap.
Use sterile vials containing 3 mL of sterile M4 media. If using swabs, use only sterile Dacron or Rayon swabs.
Do not use calcium alginate swabs, as they may contain substances that inhibit PCR testing. Break applicator stick off near the tip to permit tightening of the cap. Sputum: Collect in sputim collection kit or a sterile, plastic container with a leak-proof cap. Bronchial Lavage/wash: Collect in a sterile container with a leak-proof cap. Respiratory Samples in M4 media: Use sterile vials containing 3 mL of sterile M4 media. If using swabs, use only sterile Dacron or Rayon swabs. Do not use calcium alginate swabs, as they may contain substances that inhibit PCR testing. Break applicator stick off near the tip to permit tightening of the cap. Sputum: Collect in sputim collection kit or a sterile, plastic container with a leak-proof cap. Bronchial Lavage/wash: Collect in a sterile container with aleak-proof cap. Nasopharyngeal aspirate/wash: Collect in a sterile container with a leak-proof cap. Tracheallavage/wash: Collect in a sterile container with a leak-proof cap.
Transport Container
Throat swab or Nasopharyngeal swab
__Multi Microbe Media (M4) (preferred)
__V-C-M medium (green-cap) tube or equivalent (UTM) (preferred)
__V-C-M medium (green-cap) tube or equivalent (UTM) (preferred)
Sputum or Bronchial lavage/wash or Nasopharyngeal aspirate/wash or Tracheal lavage/wash: Sterile leak-proof container
__Multi Microbe Media (M4) (preferred)
__V-C-M medium (green-cap) tube or equivalent (UTM) (preferred)
__V-C-M medium (green-cap) tube or equivalent (UTM) (preferred)
Sputum or Bronchial lavage/wash or Nasopharyngeal aspirate/wash or Tracheal lavage/wash: Sterile leak-proof container
Transport Temperature
All specimen types listed: Refrigerated preferred; Room
_temperature unacceptable; Frozen acceptable
_temperature unacceptable; Frozen acceptable
Specimen Stability
Throat swab or Nasopharyngeal swab or Sputum or Bronchial
lavage/wash or Nasopharyngeal aspirate/wash or Tracheal
lavage/wash
__Room temperature: 48 Hours
__Refrigerated: 7 Days
__Frozen: 30 Days
lavage/wash or Nasopharyngeal aspirate/wash or Tracheal
lavage/wash
__Room temperature: 48 Hours
__Refrigerated: 7 Days
__Frozen: 30 Days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Failure to follow collection instructions.
Methodology
Real-Time Polymerase Chain Reaction
Setup Schedule
Sets up 6 days a week.
Report Available
Within 72 hours
Clinical Significance
This test is used to determine the presence of respiratory virus RNA/DNA in a patient's specimen. PCR provides more rapid results than other methods, including culture. The use of a panel for virus detection provides a useful differntial diagnosis.
