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Vitamin D, 1,25-Dihydroxy, LC/MS/MS
Test CodeLAB536
Alias/See Also
VITD2, Vitamin D, 1,25-(OH), 16558
CPT Codes
82652
Includes
Vitamin D, 1,25-Dihydroxy, LC/MS/MS
Preferred Specimen
2 mL Serum
Minimum Volume
1.1 mL Serum
Other Acceptable Specimens
Red-top (no gel) (preferred)
SST (red-top)
SST (red-top)
Instructions
Collect blood in a non-gel barrier red-top tube. Allow blood to clot (30 minutes) at room temperature. Centrifuge and separate the serum from the cells. Note: If sample is submitted with less than 1.1 mL and needs to be repeated, the sample will be canceled with the comment TNP-Initial testing necessitated a repeat, but there was insufficient sample to perform repeat.
Transport Container
Red-top (no gel) (preferred)
SST (red-top)
SST (red-top)
Transport Temperature
Serum: Room temperature preferred; Refrigerated acceptable;
Frozen acceptable
Frozen acceptable
Specimen Stability
Serum
Room temperature: 28 Days
Refrigerated: 28 Days
Frozen: 28 Days
Room temperature: 28 Days
Refrigerated: 28 Days
Frozen: 28 Days
Methodology
Liquid Chromatography Tandem Mass Spectrometry
Setup Schedule
Sets up 7 days a week, Nightly
Report Available
Reports in 3 days.
Clinical Significance
This test measures the bioactive form of vitamin D. It is used in the differential diagnosis of hypocalcemia and to monitor patients with renal osteodystrophy or chronic renal failure. This test is not suitable for diagnosis of vitamin D deficiency and monitoring supplementation in most patients. The 25 hydroxyvitamin D test is the recommended test for those purposes (N Engl J Med. 2007;357:266-281).
Additional Information
LMRP_Vitamin_D_L36692
