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Testosterone, Free and Total
MessageAids in the evaluation of suspected hypogonadism in men with a total testosterone level at the lower limit of the normal range and the detection of hyperandrogenemia in women.
Test Code
LAB173
Alias/See Also
Free and Total Testosterone, for
Adult Males and Females
Adult Males and Females
Includes
Albumin
Sex Hormone Binding Globulin
Testosterone Total
Testosterone Free
Sex Hormone Binding Globulin
Testosterone Total
Testosterone Free
Minimum Volume
Albumin = 0.2 mL plasma
Testosterone, SHBG = 0.5 mL serum
Testosterone, SHBG = 0.5 mL serum
Instructions
Routine venipuncture.
Collect two 5 mL Red/Gold top serum separator tubes.
Collect two 5 mL Red/Gold top serum separator tubes.
Specimen Stability
Albumin = Refrigerate, stable 48 hours; stable 8 hours at room temperature; stable >48 hours frozen
SHBG = Refigerate, stable 7 days
SHBG = Refigerate, stable 7 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
insufficient quantity; hemolysis or lipemia; failure to follow stability requirements
Methodology
Albumin = Dye binding - BCG
Testosterone Total = Electrochemiluminescence immunoassay
SHBG = Electrochemiluminescence immunoassay
Testosterone free = calculated
Setup Schedule
Tuesday, Friday - PM Shift
Report Available
Within 24 hours of setup run
Limitations
TSTFC = Testosterone, Free cannot be ordered on a pediatric
patient less than 18 years old.
patient less than 18 years old.
Reference Range
Albumin 3.5 - 5.0 g/dL
SHBG
Female 18-144 nmol/L
Male 10-57 nmol/L
Testosterone Total
Females:
Ovulating: 0-73 ng/dL
Post Menopausal: 0-43 ng/dL
Males:
20-49 years: 72-853 ng/dL
>50 years: 129-767 ng/dL
Testosterone Free
Female => 18 years old 0.328 - 2.250 %
Male => 18 years old 1.470 - 3.210 %
SHBG
Female 18-144 nmol/L
Male 10-57 nmol/L
Testosterone Total
Females:
Ovulating: 0-73 ng/dL
Post Menopausal: 0-43 ng/dL
Males:
20-49 years: 72-853 ng/dL
>50 years: 129-767 ng/dL
Testosterone Free
Female => 18 years old 0.328 - 2.250 %
Male => 18 years old 1.470 - 3.210 %