P2Y12 Platelet Function

Test Code

LAB2313

Alias/See Also

Ticagrelor Platelet Function Test
Ticlopidine (Ticlid) Platelet Function Test
Plavix Platelet Function Test
Verify Now P2Y12
P2Y12 Platelet Function Test
Clopidogrel (Plavix) Platelet Function Test
Thienopyridine Platelet Function Test
Prasurgrel Platelet Function Test
Brilinta (Ticagrelor)

CPT Codes
85576

Preferred Specimen

2-mL Griener Bio-One Vacuette tubes (whole blood Sodium Citrate Plasma)
2 Full tubes and 21 gauge or larger needle should be use
Do Not centrifuge

Patient Preparation
Manufacturer suggested test timing based on therapy and dose:
Clopidogrel (Plavix®) 75 mg ≥ 7 days on maintenance
Clopidogrel (Plavix®) 300 mg ≥ 8 hours post-bolus
Clopidogrel (Plavix®) 600 mg ≥ 6 hours post-bolus
Prasugrel (Effent®) 5 mg ≥ 5 days on maintenance
Prasugrel (Effent®) 10 mg ≥ 5 days on maintenance
Prasugrel (Effent®) 60 mg ≥ 45 minutes post-bolus
Ticagretor (Brilinta®) 90 mg (bid) ≥ 1 day (within 8 hours of last dosr maximal effect)Ticagretor (Brilinta®) 180 mg ≥ 2 hours post-bolus (within 8 hours for maximal effect)

Minimum Volume

2mL full tubes

Instructions

ttention to collection procedure is necessary to avoid inaccurate results, collect as follows:
Draw a discard tube (at least 2 mL).
Draw 2 Griener blue-top tubes. Do not overfill or underfill.
Gently invert the tube at least 5 times to ensure complete mixing of the contents
Forward promptly at Room temperature.

Special Handling
DO NOT SEND THROUGH PNEUMATIC TUBE SYSTEM.

Transport Container

Primary Container.

Transport Temperature

Room Temp

Specimen Stability

Whole blood specimen should arrive at lab within 3 hours of collection.

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Specimens will be rejected if not properly filled, clotted, grossly hemolyzed, or contaminated with heparin, or sent through the pneumatic tube system.

Methodology
Whole Blood aggregometry (platelet inhibition) VerifyNow P2Y12 (PRU) assay

Setup Schedule

Daily, Stat as needed

Report Available

1-2 hours

Limitations

Test performance was not affected by hematocrit values between 33-52%, or platelet count values between 119,000 -502,000/µL.

Patients with inherited platelet disorders such as von Willebrand Factor Deficiency, Glanzmann Thrombasthenia and Bernard-Soulier Syndrome have not been studied with the VerifyNow PRUTest. The VerifyNow PRUTest is not intended for use with these types of platelet disorders.

Patients who have been treated with Glycoprotein IIb/IIIa inhibitor drugs should not be tested until platelet function has recovered. This time period is approximately 14 days after discontinuation of drug administration for abciximab (ReoPro)and up to 48 hours for eptifibatide (Integrilin) and tirofiban (Aggrastat). The platelet function recovery time varies
among individuals and is longer for patients with renal dysfunction.

Reference Range

180 - 376 PRU. Values less than 180 PRU may be evidence of a P2Y12 inhibitor effect.

Clinical Significance

The VerifyNow PRUTest is designed to measure platelet P2Y12 receptor blockade. Substances known to specifically
block the P2Y12 receptor include the thienopyridine class of drugs, including clopidogrel.

Performing Laboratory
CHOMP Laboratory

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.