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Antibody Screen
Test CodeLAB278
Alias/See Also
Indirect Coombs; Ab Screen
CPT Codes
86850
Instructions
Blood by routine venipuncture in pink top (EDTA) tube (preferred) or blood in lavender top (EDTA) tube and blood in red top, no-additive clot tube.
Specimen Stability
7 mL whole blood at 20-25°C. (Min: 3 mL)
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Improperly labeled specimen; gross hemolysis or lipemia; container damaged and leaking; improper transport (too hot/partially frozen red cells); specimen older than 48 hours unrefrigerated; insufficient quantity; bacterial contamination
Methodology
Antigen/antibody reaction
Setup Schedule
Daily
Report Available
Routine outpatient: 8-24 hours
Clinical Significance
Use to detect in vitro sensitization of RBCs. The presence of alloantibodies, autoantibodies, and complement components can be detected by antihuman globulin serum in the antiglobulin test.
