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Shiga Toxin Stool
Test CodeLAB2002
Alias/See Also
Shiga Like Toxin, Stool
CPT Codes
87427 x2
Includes
Shiga Toxin 1 and Shiga Toxin 2
Preferred Specimen
Stool
Minimum Volume
1 g or 1 mL
Instructions
OUTPATIENT:
Two red cap ETM vials are provided, 1 Room Temperature and 1 Refrigerated.
Preferred specimen: Stool
1) Stool specimens should be passed into a dry, clean container, the stool must NOT be contaminated with urine or water
2) Slimy, bloody, or watery stool, if present, should be selected and placed into each vial. Place enough specimen to reach the FILL LINE.
3) Carefully break up the specimen and swirl the contents thoroughly using the spork (attached to the cap). Screw on and tighten the cap without further mixing or shaking.
4) Label each vial (Room Temperature and Refrigerated) with appropriate patient information including name, date and time of collection.
INPATIENT:
Preferred specimen: Stool
Minimum volume: 1 g or 1 ml
Stool specimens should be passed into a dry, clean container, the stool must NOT be contaminated with urine or water
Instructions: Collect stool in clean container and deliver to lab immediately, OR
Place enough stool into enteric (red top vial) preservative vial to bring level of liquid to indicated fill line. Label, mix well, and send to the laboratory immediately.
Transport container: Clean container -ETM (Enteric Transport Medium) transport medium, red cap vial
Two red cap ETM vials are provided, 1 Room Temperature and 1 Refrigerated.
Preferred specimen: Stool
1) Stool specimens should be passed into a dry, clean container, the stool must NOT be contaminated with urine or water
2) Slimy, bloody, or watery stool, if present, should be selected and placed into each vial. Place enough specimen to reach the FILL LINE.
3) Carefully break up the specimen and swirl the contents thoroughly using the spork (attached to the cap). Screw on and tighten the cap without further mixing or shaking.
4) Label each vial (Room Temperature and Refrigerated) with appropriate patient information including name, date and time of collection.
INPATIENT:
Preferred specimen: Stool
Minimum volume: 1 g or 1 ml
Stool specimens should be passed into a dry, clean container, the stool must NOT be contaminated with urine or water
Instructions: Collect stool in clean container and deliver to lab immediately, OR
Place enough stool into enteric (red top vial) preservative vial to bring level of liquid to indicated fill line. Label, mix well, and send to the laboratory immediately.
Transport container: Clean container -ETM (Enteric Transport Medium) transport medium, red cap vial
Transport Container
Inpatient: Collect stool in clean container and deliver to lab immediately or
Inpatient and outpatient: Clean container -ETM (Enteric Transport Medium) transport medium, red cap vial
Inpatient and outpatient: Clean container -ETM (Enteric Transport Medium) transport medium, red cap vial
Transport Temperature
OUTPATIENT:Two red cap ETM vials are provided, 1 Room Temperature and 1 Refrigerated.
INPATIENT: Room Temperature
INPATIENT: Room Temperature
Specimen Stability
Inpatient: Fresh stool at room temperature up to 1 hour.
Specimen in enteric preservative up to 5 days.
Specimen in enteric preservative up to 5 days.
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Outpatient:
Frozen stool; unacceptable preservative vial; fresh stool that is greater than 1 hour old; Rectal Swab, leaking container; overfilled preservative vial; urine or barium contamination; more than one stool culture per day.
Inpatient: Refrigerated or frozen stool; unacceptable preservative vial; fresh stool that is too old;Rectal Swab, leaking container; overfilled preservative vial; urine or barium contamination; more than one stool culture per day
Frozen stool; unacceptable preservative vial; fresh stool that is greater than 1 hour old; Rectal Swab, leaking container; overfilled preservative vial; urine or barium contamination; more than one stool culture per day.
Inpatient: Refrigerated or frozen stool; unacceptable preservative vial; fresh stool that is too old;Rectal Swab, leaking container; overfilled preservative vial; urine or barium contamination; more than one stool culture per day
Methodology
Enzyme Immuno Assay
Setup Schedule
Daily
Report Available
1 day
Limitations
The molecular assay uses nucleic acid amplification to detect toxigenic C. difficile. It is at least 95% sensitive and specific. Given the high sensitivity, repeat testing after a negative result is not advised. It should not be used as a test for cure.
Reference Range
Negative
Performing Laboratory
Community Hospital of the Monterey Peninsula Laboratory
