|
|
| A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
VITAMIN D 25, TOTAL
MessageThis is the primary test used to identify vitamin D insufficiency.
Test Code
LAB123264
CPT Codes
82306
Preferred Specimen
Adult: One Gold Top Tube
Pediatric: 2 Gold Top Microtainers
Pediatric: 2 Gold Top Microtainers
Minimum Volume
0.5mL Serum
Other Acceptable Specimens
One Red Top Tube
Instructions
Centrifuge within 1 hour of collection. Separate serum from cells if Red Top Tupe. Aliquot 0.5mL serum
Transport Temperature
Refrigerated
Specimen Stability
Ambient: 72 hours
Refrigerated: 12 days
Frozen: 1 Year
Refrigerated: 12 days
Frozen: 1 Year
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Moderate or Gross Hemolysis, Gross Lipemia
Methodology
Chemiluminescent Immunoassay
Reference Range
20-100 ng/mL
Per Endocrine Society:
<10 ng/mL: severe deficiency, could be assoicated with osteomalacia or rickets.
10-19 ng/mL: mild to moderate deficiency, might be associated with increased risk of osteoporosis or secondary hyperparathyroidism
>100 ng/mL: toxicity possible, most patients with toxicity have levels > 150ng/mL
The optimal levels of vitamin D are not well establised and remain controversial. The 2024 Endocrine Soceity Clinical Practice Guideline no longer considers 25 (OH) D levels of 20-29 insufficient for generally healthy individuals (PMID: 38828961 and 38828931). Desirable vitamin D levels may differ in those with disorders of calcium homeostatis and/or metabolic bone disease.
Per Endocrine Society:
<10 ng/mL: severe deficiency, could be assoicated with osteomalacia or rickets.
10-19 ng/mL: mild to moderate deficiency, might be associated with increased risk of osteoporosis or secondary hyperparathyroidism
>100 ng/mL: toxicity possible, most patients with toxicity have levels > 150ng/mL
The optimal levels of vitamin D are not well establised and remain controversial. The 2024 Endocrine Soceity Clinical Practice Guideline no longer considers 25 (OH) D levels of 20-29 insufficient for generally healthy individuals (PMID: 38828961 and 38828931). Desirable vitamin D levels may differ in those with disorders of calcium homeostatis and/or metabolic bone disease.
Performing Laboratory
West Virginia University Hospital, Inc.
Berkeley Medical Center
Jefferson Medical Center
