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COOMBS, DIRECT (DAT)
Test CodeLAB274
CPT Codes
86880
Preferred Specimen
One 6 mL lavender top tube
Pediatric: One lavender top microtainer
Pediatric: One lavender top microtainer
Minimum Volume
3 mL blood (Adult)
Instructions
Please refer to Transfusion Practice Policy for further information on transfusion practices.
Patient’s red cells are tested using polyspecific antihuman globulin antisera (anti-IgG and C3). If positive, then test will be performed using monospecific reagents specific for IgG and C3. An elution and antibody identification will be performed on positive DAT specimens.
Received in Sunquest by Blood Bank only.
Patient’s red cells are tested using polyspecific antihuman globulin antisera (anti-IgG and C3). If positive, then test will be performed using monospecific reagents specific for IgG and C3. An elution and antibody identification will be performed on positive DAT specimens.
Received in Sunquest by Blood Bank only.
Transport Temperature
Refrigerated
Specimen Stability
Refrigerated: 24 hours
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Mislabeled specimen
Methodology
Hemagglutination/Hemolysis
Setup Schedule
Sunday-Saturday
Clinical Significance
This test is used to identify in-vivo coating of RBCs with IgG and/or the complement component C3d which can occur in conditions
including autoimmune hemolytic anemia, hemolytic transfusion reactions, and drug-induced hemolytic anemia.
Interpretation:
Negative: No IgG antibody or complement (C3d) detected on the surface of the red cell.
Positive: IgG or complement (C3d) is present on the surface of the red cell.
Cautions:
- Autoimmune hemolytic anemia can occur with a negative direct Coombs test.
- The direct Coombs test can be positive in some apparently healthy individuals.
including autoimmune hemolytic anemia, hemolytic transfusion reactions, and drug-induced hemolytic anemia.
Interpretation:
Negative: No IgG antibody or complement (C3d) detected on the surface of the red cell.
Positive: IgG or complement (C3d) is present on the surface of the red cell.
Cautions:
- Autoimmune hemolytic anemia can occur with a negative direct Coombs test.
- The direct Coombs test can be positive in some apparently healthy individuals.
Performing Laboratory
West Virginia University Hospital, Inc.
Berkeley Medical Center
Braxton County Memorial
Camden Clark Medical Center
Garrett Regional Medical Center
Jefferson Medical Center
Princeton Community Hospital
Potomac Valley Hospital
Reynolds Memorial Hospital
Summersville Regional Medical Center
St. Joseph’s Hospital
United Hosiptal Center
Uniontown Hospital
Wetzel County Hospital
