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PTT (ACTIVATED PARTIAL THROMBOPLASTIN TIME)
MessageA hematocrit value >55% may lead to spurious coagulation results.
Test Code
LAB325
CPT Codes
85730
Preferred Specimen
One 3 mL blue top tube
Pediatric: One 1.8 mL pediatric blue top tube
Pediatric: One 1.8 mL pediatric blue top tube
Minimum Volume
1 mL plasma
Instructions
Tube must be filled to the etched line. Immediately after drawing gently invert the specimen 3 to 4 times. Do not shake.
Samples must be centrifuged at 3000 rpm for 10 minutes or using a high-speed centrifuge. Platelet poor plasma should be separated into 1 ml aliquots. Samples should be frozen within one hour (best practice), however samples stable for 4 hours. Samples must remain frozen during transport. Any samples that are received thawed are not suitable for testing.
Samples must be centrifuged at 3000 rpm for 10 minutes or using a high-speed centrifuge. Platelet poor plasma should be separated into 1 ml aliquots. Samples should be frozen within one hour (best practice), however samples stable for 4 hours. Samples must remain frozen during transport. Any samples that are received thawed are not suitable for testing.
Transport Temperature
Frozen (1 mL plasma aliquot)
Specimen Stability
Ambient: 4 hours if cap has not been removed or punctured
Frozen: 6 months (-70C)
Frozen: 6 months (-70C)
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Clotted specimen, underfilled tube, or gross hemolysis.
Methodology
Optical Density on ACL TOP using SynthASil aPTT reagent
Setup Schedule
Sunday-Saturday
Reference Range
24.2 - 37.5 sec
Note: The upper reference range limit may be higher in newborns (e.g. 40-45 sec)
Automatically Called Results > 120.0 sec
No Result: Analyzer Fails to obtain results. No Clotting deteced during testing. Please repeat if clinically indicated.
Note: The upper reference range limit may be higher in newborns (e.g. 40-45 sec)
Automatically Called Results > 120.0 sec
No Result: Analyzer Fails to obtain results. No Clotting deteced during testing. Please repeat if clinically indicated.
Clinical Significance
Interpretive Information
Therapeutic range for unfractionated heparin is 60.0 to 100.0 seconds.
Direct oral anticoagulants can effect coagulation testing results.
Therapeutic range for unfractionated heparin is 60.0 to 100.0 seconds.
Direct oral anticoagulants can effect coagulation testing results.
Performing Laboratory
West Virginia University Hospital, Inc.
Berkeley Medical Center
Braxton County Memorial Hospital
Camden Clark Medical Center
Fairmont Medical Center
Jackson General Hospital
Jefferson Medical Center
Potomac Valley Hospital
Reynolds Memorial Hospital
Summersville Regional Medical Center
St Joseph's Hospital
United Hospital Center
Wetzel County Hospital
