| A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
PT Mixing Study
MessagePerformed at BUMCT-Coagulation
Test Code
PT MIX/T
CPT Codes
85611
Preferred Specimen
3.2% Sodium Citrate – Blue Top Tube
Instructions
BUMCS - double spin technique to obtain platelet poor plasma.
North Clinic and Orange Grove Clinic - if sample to arrive at BUMCT within 4 hours may send whole blood.
Centrifuge within 1 hour of collection. If testing is not complete within 4 hours of collection, Remove plasma using plastic transfer pipettes and double spin technique, freeze in plastic tube
North Clinic and Orange Grove Clinic - if sample to arrive at BUMCT within 4 hours may send whole blood.
Centrifuge within 1 hour of collection. If testing is not complete within 4 hours of collection, Remove plasma using plastic transfer pipettes and double spin technique, freeze in plastic tube
Transport Temperature
| · <4 of collection - room temperature |
| · >4 hours of collection -Frozen-use dry ice |
Specimen Stability
| · Plasma stability - 4 hours at 2-8°C or 20°C, |
| · 2 weeks at -20°C - -80°C in a non-frost-free freezer |
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
| · clotted specimens |
| · underfilled or overfilled tube |
| · collection in 3.8% Citrate |
| · specimens collected in expired tubes |
| · serum samples |
| · specimen past stability |
| · thawed frozen samples |
| · Hemolyzed, Icteric and Lipemic samples |
| · Hct >55% |
Reference Range
PT 9.4 – 12.5 seconds
Clinical Significance
In the event of an unexplained prolonged PT and/or PTT, it is clinically necessary to determine if the long clotting time is due to a factor deficiency or to the effects of a circulating inhibitor (antibody).
