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Group B Strep PCR
MessagePerformed at BUMCT
NOTE: CDC Recommendations:
Collection of cultures between 35 and 37 weeks gestation is recommended to improve the sensitivity and specificity of detection of women who remain colonized at the time of delivery. Swabbing both the lower vagina and rectum (i.e., through the anal sphincter) increases the yield substantially compared with sampling the cervix or sampling the vagina without also swabbing the rectum.
NOTE: CDC Recommendations:
Collection of cultures between 35 and 37 weeks gestation is recommended to improve the sensitivity and specificity of detection of women who remain colonized at the time of delivery. Swabbing both the lower vagina and rectum (i.e., through the anal sphincter) increases the yield substantially compared with sampling the cervix or sampling the vagina without also swabbing the rectum.
Test Code
GRPBPCR
Alias/See Also
Genital Screen by PCR method for Group B Streptococcus
Preferred Specimen
Specimen collected on eSwab from combined vaginal introitus and anorectal areas.
Other Acceptable Specimens
Specimen collected on eSwab or Copan red top swab from combined vaginal introitus and anorectal areas.
Specimen Stability
eSwabs or Copan red top swab: Up to 24 hours, if refrigerated 2 to 8oC
Methodology
DiaSorin Simplexa
Setup Schedule
Swabs undergo preenrichment culture in LIM broth prior to PCR.
Incubated at 1400 and 2000 hours for 18-24hrs.
Incubated at 1400 and 2000 hours for 18-24hrs.
Report Available
Tested daily after 18-24hr incubation.
Clinical Significance
Qualitative detection of Group B Streptococcus (GBS) to screen and determine GBS colonization status of pregnant women at 35 to 37 weeks of gestation.
