| A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
Heparin Induced Platelet Antibody IgG
MessagePerformed at BUMCT
Test Code
HITE
Preferred Specimen
Plasma collected in: 3.2% sodium citrate (light blue-top) tube
Instructions
Light blue capped tubes (3.2% buffered sodium citrate) used for coagulation testing must be allowed to fill completely (after filling a small air space will normally be present). Short filled tubes will be rejected.Centrifuge within 1 hour of collection. If testing is not performed on day of collection transfer specimen to polypropylene plastic tube using a plastic transfer pipette. (Pour off 2 plasma aliquots). Freeze plasma < -20°C.
Specimen Stability
| · 8 hours at 2-8°C |
| · 2 -3 years at -20°C or below , in a non-frost-free freezer |
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
| · clotted specimens |
| · underfilled or overfilled tube |
| · collection in 3.8% Citrate |
| · specimens collected in expired tubes |
| · serum samples |
| · specimen past stability |
| · thawed frozen samples |
| · Hemolyzed, icteric, lipemic sample, heat-inactivated, or microbially contaminated specimens |
| · Diluted samples |
Methodology
Immucor PF4 IgG ELISA assay
Setup Schedule
Mon - Wed - Fri (except holidays)
Note - specimen deadline is 10am on day of run
Note - specimen deadline is 10am on day of run
Report Available
By 1600 on the day of run
Clinical Significance
Heparin Induced Thrombocytopenia (HIT) is a serious immune-mediated complication of heparin therapy affecting 1 –
4% of patients receiving the drug. HIT occurs when IgG antibodies are formed against a neoantigen created by
complexes of heparin and platelet factor 4. These antibodies activate platelets, resulting in thrombocytopenia and
thrombosis.
4% of patients receiving the drug. HIT occurs when IgG antibodies are formed against a neoantigen created by
complexes of heparin and platelet factor 4. These antibodies activate platelets, resulting in thrombocytopenia and
thrombosis.
