| A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
Plavix (P2Y12) Platelet Function Test
MessagePerformed in Coagulation at BUMCP, BDMC and BBMC.
Note: Call Laboratory for special tubes and instructions.
BTMC and BEMC send to BUMCP - STAT Courier on site BEFORE collection.
Note: Call Laboratory for special tubes and instructions.
BTMC and BEMC send to BUMCP - STAT Courier on site BEFORE collection.
Test Code
P2Y12
Alias/See Also
Plavix (Clopidogrel)
Ticlid (Ticlopidine)
Effient (Prasugrel)
Verify Now
Ticlid (Ticlopidine)
Effient (Prasugrel)
Verify Now
Preferred Specimen
| 2 Greiner Bio-One Vacuette – partial fill blood collection tubes (3.2% Sodium Citrate tube) and one plain red top. *Collect 3 Greiner Blue tops if BOTH P2Y12 and Aspirin Resistance are ordered |
Instructions
| Venipuncture Collection: |
| Identify a site that is free of any peripheral venous infusions. |
| Apply the tourniquet and identify the phlebotomy site. |
| Release the tourniquet and cleanse the phlebotomy area. |
| Reapply the tourniquet just before collection. It is important to minimize the amount of time that the tourniquet is applied prior to filling the tube in order to avoid activating the platelets while obtaining the sample. |
| A 21 gauge needle or larger must be used to avoid hemolysis. |
| Collect specimens in the following order: A Plain red top with at least 2 mL of whole blood must be drawn prior to drawing the Special Greiner Blue top tubes. Special Greiner Blue top tubes: If a single test is ordered: Collect 2 (Two) Special Greiner Blue top tubes. If both Aspirin Resistance Test and P2Y12 are ordered: Collect 3 (Three) Special Greiner Blue top tubes. (Note: the Special Greiner Blue top tube partially fills with 2.0 mL of blood, indicated by small black line. Do Not Overfill.) Note: If a CBC is to be collected at the same time, the CBC should be drawn last. |
| Immediately gently invert the blue top tube 5 times. |
| Label the red and blue top tubes with Cerner labels. Write the collection time and collector ID on each tube. |
| All samples must be hand delivered to the lab (DO NOT SEND SAMPLES THROUGH THE TUBE SYSTEM). |
| All samples must be kept at room temperature. |
| Central Line Collection: |
| Ensure that the Central line is free of clots. |
| Obtain a discard of at least 5 mL in order to clear the line. |
| Obtain the sample for testing with a second syringe. |
| Immediately transfer in the following order: 2.0 mL of blood into a plain red top tube Special Greiner Blue top tube: If a single test is ordered: Collect 2 (Two) Special Greiner Blue top tubes. If both Aspirin Resistance Test and P2Y12 are ordered: Collect 3 (Three) Special Greiner Blue top tubes (Note: the Special Greiner Blue top tube partially fills with 2.0 mL of blood, indicated by small black line. Do Not Overfill.) |
| Immediately gently invert the blue top tube 5 times. |
| Label the red and blue top tubes with Cerner labels. Write the collection time and collector ID on each tube. |
| All samples must be hand delivered to the lab (DO NOT SEND SAMPLES THROUGH THE TUBE SYSTEM). |
| All samples must be kept at room temperature. |
Transport Container
AZ Valley locations send to BUMCP Ambient
Specimen Stability
4 hours at room Temperature
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hand deliver all 3 tubes to the lab within one hour of collection. DO NOT send through the telelift system.
| · Clotted, hemolyzed, underfilled or overfilled (blood must be filled to the black line), BD Sodium Citrate Blue Top tube, Drawn in outdated/expired tube. |
| · Heated, refrigerated, or frozen |
| · Centrifuged |
| · Delivered by pneumatic tube |
| · Past stability |
Report Available
BUMCP- Report available within 4 hours from receipt at testing facility.
Reference Range
Units of Measure = PRU
Off drug: 180 - 376 PRU
Values less than 180 PRU suggests evidence of a P2Y12 inhibitor effect.
Off drug: 180 - 376 PRU
Values less than 180 PRU suggests evidence of a P2Y12 inhibitor effect.
Clinical Significance
The Verify Now PRU Test is a whole blood assay used in the laboratory to measure the level of platelet P2Y12 receptor
blockade.
blockade.
