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Acanthamoeba Culture
MessagePerformed in Microbiology
Test Code
C ACANTH
Includes
Culture,
Calcofluor stain, if applicable
Calcofluor stain, if applicable
Preferred Specimen
Excised cornea, corneal scrapings, or biopsy
Note: Tissue samples must be eye related and should be in sterile containers
with 2ml of sterile saline to prevent specimen from drying out
Swab of conjunctiva or corneal ulcer
Note: Swab transport with modified Stuart's or Amies
transport media, or eSwabs
Contact lenses in lens solution or in a sterile container
Note: Tissue samples must be eye related and should be in sterile containers
with 2ml of sterile saline to prevent specimen from drying out
Swab of conjunctiva or corneal ulcer
Note: Swab transport with modified Stuart's or Amies
transport media, or eSwabs
Contact lenses in lens solution or in a sterile container
Instructions
Requires a dedicated sample for acanthamoeba culture.
Transport Temperature
Room temperature (ambient)
Specimen Stability
For Tissues: Send to lab within 30 mins. of collection
For all other specimens: Send to lab as soon as possible.
Specimens are stable at room temperature < 48 hours
DO NOT refrigerate specimens.
DO NOT place specimens on ice.
For all other specimens: Send to lab as soon as possible.
Specimens are stable at room temperature < 48 hours
DO NOT refrigerate specimens.
DO NOT place specimens on ice.
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Unacceptable specimens:
Dry swabs
Brain tissue
Water samples
Any other specimens received other than those described above.
Dry swabs
Brain tissue
Water samples
Any other specimens received other than those described above.
Performing Laboratory
BUMCT
