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Legionella Culture
Test CodeC LEG
Preferred Specimen
Lower respiratory tract specimens including bronchial washings, bronchoalveolar lavage, bronchial brushings,
induced or expectorated sputums, endotracheal or nasotracheal suctions, tracheal aspirates
Sterile fluid such as pleural fluid, peritoneal or pericardial fluid
Lung tissue
Blood
induced or expectorated sputums, endotracheal or nasotracheal suctions, tracheal aspirates
Sterile fluid such as pleural fluid, peritoneal or pericardial fluid
Lung tissue
Blood
Minimum Volume
1 ml
Instructions
All of the above specimens should be collected in sterile, screw-capped container.
Blood (collect 6-10ml in a SPS (sodium polyanethol sulfonate) tube)
Blood (collect 6-10ml in a SPS (sodium polyanethol sulfonate) tube)
Transport Temperature
For Respiratory Specimens: Keep refrigerated.
For Tissue Samples: Keep refrigerated.
DO NOT leave at room temperature.
DO NOT freeze
For Tissue Samples: Keep refrigerated.
DO NOT leave at room temperature.
DO NOT freeze
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Unacceptable specimens:
Specimens with excessive transport time
Specimen types other than those described above
NOTE: for environmental testing for Legionella, refer to Infection Preventionist for further information.
Environmental testing not performed by hospital laboratories.
Specimens with excessive transport time
Specimen types other than those described above
NOTE: for environmental testing for Legionella, refer to Infection Preventionist for further information.
Environmental testing not performed by hospital laboratories.
Clinical Significance
Bacteria in the genus Legionella primarily cause respiratory illness, i.e. either Legionnaires' Disease, a systemic illness manifested primarily by pneumonia, or Pontiac fever, a nonpnuemonic, influenza-like illness. Legionellae may also cause a variety of other illnesses.
Performing Laboratory
BUMCT