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Fungal Smear (Calcofluor Stain)
MessageLab only orderable. Unit will have to put in a Lab Communication
Calcofluor stain for fungal elements (mold) or Coccidioides species spherules
Calcofluor white stains are automatically performed for the following sources, when a fungal culture is ordered:
Calcofluor (fungal) stains maybe requested as a separate Lab Communication for the following sources
Calcofluor stain for fungal elements (mold) or Coccidioides species spherules
Calcofluor white stains are automatically performed for the following sources, when a fungal culture is ordered:
- Bronchoalveolar Lavage
- Corneal scraping
- Liquid specimens such as abscess fluids, eye fluids
- Sterile body fluid
- Tissue
Calcofluor (fungal) stains maybe requested as a separate Lab Communication for the following sources
- bronchial brushing
- bronchial washing
- hair, skin, nail
- Esophageal brushing
- tongue scrapings
Test Code
CALC
Alias/See Also
Fungal stain
Fungal smear
Calcofluor Candida Thrush Screen (MD Anderson ONLY)
Fungal smear
Calcofluor Candida Thrush Screen (MD Anderson ONLY)
CPT Codes
87206
Preferred Specimen
Lower respiratory specimens such as: Bronchoalveolar lavage, Lung tissue
Sterile body fluids, tissue samples, abscess aspirates, corneal scrapings
Sterile body fluids, tissue samples, abscess aspirates, corneal scrapings
Instructions
Describe specific type of specimen, such as: Bronchoalveolar lavage, Pleural fluid, Left lung tissue
Transport Temperature
Ambient/ Room Temperature.
Specimen Stability
For Fluids or Tissues: Send to the Lab within 2 hours of collection.
DO NOT refrigerate specimens.
DO NOT place specimens on ice.
DO NOT refrigerate specimens.
DO NOT place specimens on ice.
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Unacceptable specimens:
Urine specimens
Genital specimens
Specimens received on swabs including eSwabs
Urine specimens
Genital specimens
Specimens received on swabs including eSwabs
Methodology
Microscopic examination
Clinical Significance
Calcofluor white is used for rapid screening of clinical specimens for fungal elements. Calcofluor white stain is a colorless, nonspecific fluorescent dye that binds certain polysaccharides present in cellulose and chitin. It exhibits fluorescence when the dye is exposed to long wave ultraviolet light and short wavelength visible light. Clinical specimen (fresh, frozen, fixed, or paraffinimbedded) where fungus including Pneumocystis jiroveci is suspected or fungal growth isolated on culture media is suitable for examination using this test.
