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Influenza A and B Virus DFA and Culture

Test Code
C FLU


Alias/See Also
Influenza Culture and DFA
Flu Culture and DFA
SQL test code 4576


Includes
Cultured for: Influenza A and Influenza B


Preferred Specimen
Swabs in viral transport media (VTM) or universal transport media (UTM).  Acceptable sources include:
  • Nasopharyngeal (NP)
  • Nasal
  • Throat
Acceptable sources in sterile container without VTM or UTM:
  • Nasopharyngeal wash or aspirate
  • Tracheal aspirates
  • Nasal wash or aspirate
  • Bronchoalveolar lavage (BAL)
  • Bronchial lavage/wash
  • Bronchial brush


Instructions
Nasopharyngeal Swab Collection:
  • Use only swabs with synthetic tip (e.g., Dacron, nylon, or rayon) and an aluminum or plastic shaft.
  • Grasp the shaft, using the thumb and forefinger; about 3 inches from the tip.
  • Gently insert the swab into one nostril, stopping when the swab contacts the mid-inferior portion of the inferior turbinate (1/3 to 1/2 the way up the shaft).
  • Gently rotate the swab tip several times to loosen and collect the material.
  • Gently withdraw the swab and place in VTM or UTM.
  • Refrigerate and send to lab.


Transport Temperature
Refrigerated


Specimen Stability
Ambient: Unacceptable
Refrigerated: 2 days
Frozen (-70°C): 30 days


Methodology
Direct Fluorescent Antibody (DFA) test
Tissue Culture
Immunofluorescence Assay (IFA)

Setup Schedule
Days: Monday through Sunday


Report Available
DFA results available in 1 day.

If DFA result is negative or too few cells, culture is set and results are available in 2 - 3 days.


Reference Range
DFA: Negative
Culture: Negative for Virus isolation


Performing Laboratory
Sonora Quest Laboratories, Tempe



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.