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ROTEM sigma complete + hep
MessagePerformed in Coagulation
Test Code
ROTEM
Preferred Specimen
NaCit light blue top tube
Whole blood collected in a 3.2% Sodium Citrate tube - FULL TUBE required. Use 2.7 mL tube for adequate testing volume.
Whole blood collected in a 3.2% Sodium Citrate tube - FULL TUBE required. Use 2.7 mL tube for adequate testing volume.
Instructions
For samples drawn by venipuncture, the first 3 mL of blood should not be used for ROTEM testing due to the possible contaminants in the sample during normal phlebotomy. Draw a discard tube if the only sample being drawn will be for ROTEM testing.
Hand deliver sample to the lab at room temperature immediately after collection. DO NOT CENTRIFUGE.
For line draws, the first 7mL of blood should be discarded.
Hand deliver sample to the lab at room temperature immediately after collection. DO NOT CENTRIFUGE.
For line draws, the first 7mL of blood should be discarded.
Transport Temperature
Room temperature
Specimen Stability
Test immediately up to 4 hours of collection
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Whole blood samples not collected in a 3.2% sodium citrate tube
Underfilled or overfilled
Expired tube
Clotted specimens
Samples contaminated with tissue contaminants (no discard tube drawn)
Refrigerated, chilled on ice, heated or frozen
Centrifuged specimens
Sample past stability time frame
Samples sent via the tube station unless approved by the Pathologist or validation has been performed.
Hemodiluted
Underfilled or overfilled
Expired tube
Clotted specimens
Samples contaminated with tissue contaminants (no discard tube drawn)
Refrigerated, chilled on ice, heated or frozen
Centrifuged specimens
Sample past stability time frame
Samples sent via the tube station unless approved by the Pathologist or validation has been performed.
Hemodiluted
Reference Range
Clinical Significance
The ROTEM sigma includes assays that assess coagulation via the intrinsic and extrinsic pathways and aids clinicians in differentiating between surgically induced bleeding and coagulation disorder.
The single-use, multichannel ROTEM sigma cartridge contains the following assays:
The single-use, multichannel ROTEM sigma cartridge contains the following assays:
- INTEM C is used for the assessment of coagulation via the intrinsic pathway in citrated whole blood samples.
- EXTEM C is used for the assessment of coagulation via the extrinsic pathway in citrated whole blood samples.
- FIBTEM C is used for the assessment of coagulation via the extrinsic pathway in citrated whole blood samples, after blocking platelet contribution to clot firmness.
- HEPTEM C is used for the assessment of coagulation via the intrinsic pathway in citrated whole blood samples, after inactivating heparin.

