Leptospira DNA, Qualitative, Real-Time PCR

Message

This test is currently only available in Bedford CPRS. If you are a BHS provider and wish to see this test offered in BHS CPRS- please contact the BHS Laboratory. Testing performed at Quest Diagnostics.

Test Code

LEPTOSPIRA DNA,BLD(O)

CPT Codes
87798

Preferred Specimen

5 mL frozen urine collected in a sterile, plastic, leak-proof container

Minimum Volume

1 mL urine • 0.8 mL whole blood • 0.5 mL CSF

Other Acceptable Specimens

1 mL whole blood collected in an EDTA (lavender-top) tube or CSF in sterile plastic leak-proof container • 2 mL urine in Aptima®

Instructions

Urine: Collect in a sterile, plastic container with a leak-proof cap. Freeze at -20°C immediately, and transport frozen. Do not thaw.

Transport Temperature

Urine: Frozen
Whole blood/CSF: Refrigerated (cold packs)

Specimen Stability

Urine
Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 30 days

Whole blood/CSF
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days

Urine in Aptima ®
Room temperature: 5 days
Refrigerated: 7 days
Frozen: 30 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Urine received room temperature • Urine received refrigerated • Aptima® urine samples where the fluid level is not between the black fill lines

Methodology
Real-Time Polymerase Chain Reaction (RT-PCR)

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

MONDAY, WEDNESDAY, FRIDAY

Report Available

1-3 DAYS

Reference Range

Not detected

Clinical Significance

PCR can be used to rapidly diagnose Leptospirosis, an infection caused by a waterborne spirochete of the genus Leptospira. In addition, testing of blood, CSF and urine may give an indication of the stage of infection.

Last Updated: November 16, 2017

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.