Hu Antibody Screen with Reflex to Titer and Western Blot, CSF

Message

If CJD is suspected - please contact the Laboratory.

This test is currently only available in BHS CPRS. If you are a Bedford provider and wish to see this test offered in Bedford CPRS- please contact the Bedford Laboratory.

Testing performed at Quest Diagnostics.

Test Code

HU AB PANEL,CSF(O)

CPT Codes
86255

Includes

If Hu Antibody Screen, IFA, CSF is positive, then Hu Antibody, WB, CSF will be performed at an additional charge (CPT code(s): 84181).
If Hu Antibody, WB, CSF is positive, then Hu Antibody Titer, CSF will be performed at an additional charge (CPT code(s): 86256).

Preferred Specimen

3 mL CSF collected in a sterile, plastic leak-proof container

Patient Preparation
Overnight fasting is preferred

Minimum Volume

1.5 mL

Transport Temperature

Refrigerated (cold packs)

Specimen Stability

Room temperature: Unacceptable
Refrigerated: 7 days
Frozen: 6 months

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Received room temperature

Methodology
Immunofluorescence Assay (IFA) • Western Blot (WB)

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Set up: Mon, Wed, Fri; Report available: 4 days

Reference Range

Hu Ab Screen, IFA, CSF	Negative
Hu Ab, WB, CSF	Negative
Hu Ab Titer, CSF	No Hu Antibodies detected in undiluted CSF samples

Clinical Significance

Paraneoplastic Syndrome involves non-metastatic systemic effects that accompany malignant disease. Antineuronal Nuclear Antibody (Anti-Hu) is found in 5-10% of patients with small cell carcinoma of the lung. Anti-Hu is associated with subacute syndrome of encephalomyeloradiculopathy, sensory neuropathy, and autoimmune neuropathy, predominantly affecting the gastrointestinal tract.

Last Updated: September 20, 2014

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.