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Flow Specialty (Leukemia/Lymphoma)
MessageIf CJD is suspected - please contact the Laboratory
Test Code
FLOW: SPECIALTY ASSAY(LEUKEMIA/LYMPHOMA)
Alias/See Also
FLOW
Preferred Specimen
Blood: Yellow Top Tube- ACD Sol A or Lavendar EDTA
CSF: sterile container
Tissues and Fluids: RPMI (vial can be obtained from flow lab or sendouts)
Bone Marrow: Dark Green Top Tube- Sodium Heparin or Lavendar EDTA
CSF: sterile container
Tissues and Fluids: RPMI (vial can be obtained from flow lab or sendouts)
Bone Marrow: Dark Green Top Tube- Sodium Heparin or Lavendar EDTA
Instructions
Blood: Include two peripheral blood smears and CBC with Differential report
Transport Temperature
Room temperature
Specimen Stability
Room temperature --
Optimal: 24 hours
Max stability: 72 hours
Refer to Flow Cytometry General Information for additional information.
Optimal: 24 hours
Max stability: 72 hours
Refer to Flow Cytometry General Information for additional information.
Methodology
Flow Cytometry
Setup Schedule
M-F
Report Available
TAT 1 - 5 days
Limitations
Viability: If sample viability <85%, results should be interpreted with caution.
Reference Range
| Test | Reference range | Units |
| WBC | 4.5-10 | K/cmm |
| Percent lymph | 10-55 | % |
| CD3+ % | 55-84 | % |
| CD3+ # | 690-2540 | cells/cumm |
| CD3+/CD4+ % | 31-60 | % |
| CD3+/CD4+ # | 410-1590 | cells/cumm |
| CD3+/CD8+ % | 13-41 | % |
| CD3+/CD8+ # | 190-1140 | cells/cumm |
| CD4/CD8 ratio | 0.8-4.2 | |
| CD19+ % | 6-25 | % |
| CD19+ # | 90-660 | cells/cumm |
| CD56+/CD16+/CD3- % | 5-27 | % |
| CD56+/CD16+/CD3- # | 90-590 | cells/cumm |
| Viability % | >85 | % |
Performing Laboratory
Test performed in the West Roxbury Flow Cytometry Lab
Contact: Albert Filion 857-203-5942
Last Updated: September 13, 2022
