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Culture, Sputum (panel)
Test CodeCULTURE,SPUTUM(PANEL)
Alias/See Also
Sputum culture
Includes
Culture
Gram stain
Gram stain
Preferred Specimen
Sputum, Induced sputum
Minimum Volume
>1.0 mls
Instructions
Specimens should be purulent and non-salivary
S. pneumoniae isolation may improve if plating occurs within one hour of obtaining sample
Best specimens should have ≤ 10 squamous cells/10x field.
Samples that have predominantly epithelial cells will contain a comment that the specimen "Gram stain suggest poor sputum sample. Suggest repeat."
S. pneumoniae isolation may improve if plating occurs within one hour of obtaining sample
Best specimens should have ≤ 10 squamous cells/10x field.
Samples that have predominantly epithelial cells will contain a comment that the specimen "Gram stain suggest poor sputum sample. Suggest repeat."
Transport Temperature
Room temperature: ≤ 2 hours
Specimen Stability
Refrigerated 2-8 degrees C. ≤ 24 hours
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Specimens received on gauze
Setup Schedule
Daily
Report Available
TAT
48 hours
48 hours
Reference Range
Normal Upper Respiratory Flora
Performing Laboratory
Test performed in the West Roxbury Microbiology Laboratory
Contact: Scott Connolly 857-203-5954
Last Updated: August 18, 2022
