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Culture, CSF (panel)
MessageIf CJD is suspected - please contact the Laboratory immediately.
Mycology culture is automatically reflexed if the gram stain shows any segmented neutrophils or yeast present.
Mycology culture is automatically reflexed if the gram stain shows any segmented neutrophils or yeast present.
Test Code
CULTURE,CSF(PANEL)
Alias/See Also
CSF culture
Includes
CSF culture
Gram stain
Gram stain
Preferred Specimen
CSF
Minimum Volume
2.0 mls
Instructions
Lumbar puncture (LP)
a. Disinfect the puncture site with antiseptic solution and alcohol to prevent contamination and/or the introduction of infection
b. Insert needle with stylet at points ranging from L1-L2 to L4-L5 interspace. When the subarachnoid space is reached, remove the stylet; spinal fluid will appear in the needle hub.
c. Measure the hydrostatic pressure with a manometer
d. Sequentially collect CSF into a minimum of three (routinely four) sterile calibrated tubes. Additional tubes may be necessary if additional tests are required. Tube 2, 3 or 4 may be used for gram stain and culture. Tube 1 is only used for gram stain/culture if it is the only tube provided.
e. Submit immediately to the laboratory.
a. Disinfect the puncture site with antiseptic solution and alcohol to prevent contamination and/or the introduction of infection
b. Insert needle with stylet at points ranging from L1-L2 to L4-L5 interspace. When the subarachnoid space is reached, remove the stylet; spinal fluid will appear in the needle hub.
c. Measure the hydrostatic pressure with a manometer
d. Sequentially collect CSF into a minimum of three (routinely four) sterile calibrated tubes. Additional tubes may be necessary if additional tests are required. Tube 2, 3 or 4 may be used for gram stain and culture. Tube 1 is only used for gram stain/culture if it is the only tube provided.
e. Submit immediately to the laboratory.
Transport Temperature
Room temperature:
DO NOT REFRIGERATE
DO NOT REFRIGERATE
Specimen Stability
- Room temperature: ≤ 24 hours
Setup Schedule
Daily
Report Available
TAT
Gram Stain - 2 hours from arrival in laboratory.
Culture 1-5 days
Gram Stain - 2 hours from arrival in laboratory.
Culture 1-5 days
Reference Range
- No Growth on Culture
Performing Laboratory
Test performed in the West Roxbury Microbiology Laboratory
Contact: Scott Connolly 857-203-5954
Last Updated: April 29, 2026
