Alkaline Phosphatase, Heat Treated Panel

Test Code

ALKALINE PHOS,HEAT STABLE PANEL

Alias/See Also

Chemistry

Includes

Alkaline phosphatase, heat treated alkaline phosphatase

Preferred Specimen

Gold Top Tube

Minimum Volume

2 mL

Transport Temperature

Refrigerate

Specimen Stability

Room temperature: 7 days
Refrigerated (2-8°C): 7 days
Frozen (-20°C or colder): 2 months

Methodology
Kinetic Rate

Setup Schedule

M-F

Report Available

TAT 1 - 3 days

Reference Range

If <20% ALP remains after 10 minutes of heating at 56 deg C, this suggests bone origin of ALP. If 25-55% of ALP remians after 10 minutes of heating at 56 deg C, this suggests liver origin of ALP.

Clinical Significance

The isoenzymes of alkaline phosphatase can be separated according to its tissue of origin by using heat inactivation. Heating serum for 10 minutes at 56°C will inactivate most of the bone ALP; the liver iso-enzymer is slightly more resistant. Placental alkaline phosphatase iso-enzymer produced by some cancers and the placenta are particularly heat-resistant.

If <20% ALP remains after 10 minutes of heating at 56 deg C, this suggests bone origin of ALP. If 25-55% of ALP remians after 10 minutes of heating at 56 deg C, this suggests liver origin of ALP.If the percentage of heat stable alkaline phosphatase is greater than 55%, the presence of placental or Regan isoenzyme should be considered.

Performing Laboratory
Test performed at the West Roxbury Chemistry Lab
Contact: Jonathan Dryjowicz-Burek, 857-203-5973

Last Updated: April 3, 2019

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.