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Legionella DNA, Qualitative, Real-Time PCR
Test CodeLEGIONELLA DNA, QUALITATIVE RT PCR(O)
Includes
Legionella species and Legionella pneumophila
Preferred Specimen
1 mL bronchoalveolar lavage (BAL) or bronchial wash collected in a sterile plastic leak-proof container or
1 mL sputum collected in a sputum collection kit or a sterile plastic leak-proof container
1 mL sputum collected in a sputum collection kit or a sterile plastic leak-proof container
Minimum Volume
0.3 mL
Instructions
Throat, nasopharyngeal (NP) swabs: Use sterile vials containing 3 mL of sterile VCM, M4, or equivalent (UTM) media. If using swabs, use only sterile dacron or rayon swabs.
Do not use calcium alginate swabs, as they may contain substances that inhibit PCR testing. Break applicator sticks off near the tip to permit tightening of the cap.
Do not use calcium alginate swabs, as they may contain substances that inhibit PCR testing. Break applicator sticks off near the tip to permit tightening of the cap.
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Room temperature: 48 hours
Refrigerated: 14 days
Frozen: 30 days
Refrigerated: 14 days
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Calcium alginate swabs
Methodology
Real-Time Polymerase Chain Reaction
Setup Schedule
Mon-Sat
Report Available
2-3 days
Reference Range
Legionella species Not detected
Legionella pneumophila Not detected
Clinical Significance
Legionella DNA, Qualitative, Real-Time PCR - This test is intended for the qualitative detection of DNA from Legionella pneumophila and other pathogenic Legionella species in patients at risk for legionellosis.
Performing Laboratory
Quest Diagnostics
Additional Information
Click here
Last Updated: October 2, 2024
