|
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
Legionella Pneumophila Antigen, DFA
Test CodeLEGDFA
CPT Codes
87278
Preferred Specimen
1 gm fresh (unfixed) lung or other respiratory tissue, submitted in a sterile leak-proof container or
3 mL bronchial or transtracheal wash/lavage/aspirate or sputum or pleural fluid submitted in a sterile leak-proof container
3 mL bronchial or transtracheal wash/lavage/aspirate or sputum or pleural fluid submitted in a sterile leak-proof container
Minimum Volume
1 gram lung tissue or other respiratory tissue • 2 mL respiratory fluids
Instructions
Lung tissue: Place into sterile container with a small amount of sterile, non-bacteriostatic, distilled water. Do not add saline because it may be inhibitory.
Sputum: Instruct patient to gargle with water and cough deeply to collect deep respiratory specimen in sterile container.
Sputum: Instruct patient to gargle with water and cough deeply to collect deep respiratory specimen in sterile container.
Transport Container
Sterile leak-proof container
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Room temperature: Unacceptable
Refrigerated: 7 days
Frozen: Unacceptable
Refrigerated: 7 days
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Slides • Serum • Urine • Expired transport device • Environmental sources • Specimens received in viral transport media
Methodology
Direct Immunofluorescence Assay (DFA)
Setup Schedule
Set up: Mon-Sat; Report available: Next day
Clinical Significance
Bacteria in the genus Legionella primarily cause respiratory illness, i.e., either Legionnaires' Disease, a systemic illness manifested primarily by pneumonia, or Pontiac Fever, a non-pneumonic, influenza-like illness. Legionellae may also cause a variety of other illnesses.
Performing Laboratory
Quest Diagnostics Nichols Institute |
14225 Newbrook Drive |
Chantilly, VA 20153 |