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A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
Leukemia/Lymphoma Evaluation
Test CodeLAB9994
CPT Codes
88184, 88185, 88189
Includes
Initial markers to be evaluated are CD2, CD3, CD4, CD5, CD7, CD8, CD10, CD11C, CD13, CD19, CD20, CD23, CD33, CD34, CD38, CD56, CD64, CD117, HLA-DR, sKAPPA, sLAMBDA, CD45 (for gating purposes)
*Additional markers may be performed based on the pathologist review. These markers will be performed at an additional charge (CPT code(s): 88185 for each additional marker).
*Additional markers may be performed based on the pathologist review. These markers will be performed at an additional charge (CPT code(s): 88185 for each additional marker).
Preferred Specimen
3mL Lavender EDTA
Minimum Volume
Bone marrow: 1 mL
Instructions
A clinical summary or differential diagnosis is required with each specimen. If possible, submit CBC results with differential or an EDTA tube of peripheral blood. Peripheral Blood: One green, yellow, or lavender-top (sodium heparin, ACD-A, or EDTA) tube. A minimum of 3 mL is required. Tube must be kept at room temperature and shipped immediately. Bone Marrow: A minimum of 1 mL (with maximum of 4 mL to prevent hemodilution of bone marrow) submitted in a green, yellow, or lavender-top (sodium heparin, ADC-A, or EDTA) tube. The tube must be kept at room temperature and shipped immediately. Tissue: Any tissue type is acceptable. Tissue size is dependent upon leukocyte cellularity. (The tissue is disaggregated into single cells so that a minimum of 50,000 cells of interest is harvested.) Ship tissue in sterile, plastic container with RPMI 1640 enriched with FBS (10%FBS RPMI). Absolutely no fixative should be added. Refrigerate and ship immediately. Body Fluids: Any body fluid is acceptable. Sample size is dependent upon cellularity of the sample (a minimum of 50,000 cells of interest in total volume of fluid). Place fluid in sterile, plastic, leakproof container. Absolutely no fixative can be added. Refrigerate and ship immediately.
Transport Temperature
Room Temperature
Methodology
Flow Cytometry (FC)