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Protein S, Total & Free
Test CodeLAB760
CPT Codes
85305, 85306
Preferred Specimen
Blue Sodium Citrate
Minimum Volume
1 mL
Instructions
Draw blood in a light blue-top tube containing 3.2% sodium citrate, mix gently by inverting 3-4 times. Centrifuge 15 minutes at 1500 g within one hour of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial(s). Plasma must be free of platelets (<10,000/mcL). Freeze immediately and transport on dry ice.
Transport Temperature
Frozen
Specimen Stability
Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen -20°C: 30 days
Frozen -70°C: 1 year
Refrigerated: Unacceptable
Frozen -20°C: 30 days
Frozen -70°C: 1 year
Methodology
Immunoturbidimetric