HPV DNA

Test Code
LAB263


CPT Codes
87621

Preferred Specimen
8.1 mL ThinPrep in Cytyc PreservCyt Solution • Minimum 2 mL SurePath CytoRich fraction pellet re-suspended submitted in 15mL conical centrifuge tube • 2-5mm2 Biopsy specimen submitted in Digene Transport Media


Minimum Volume
ThinPrep® PreservCyt solution: 4.1 mL
SurePath™ CytoRich® fraction: 2 mL pellet re-suspended


Instructions
To submit HPV and SurePath liquid cytology testing submit a SurePath vial according to SurePath collection instructions. If only HPV testing is requested and cytology will be performed elsewhere; Following cytology slide preparation, add 2 mL of fresh SurePath fluid to the CytoRich fraction. Vortex or mix gently for 5 seconds. Cap and transport this sample to the laboratory for HPV testing. Note: Pelleted samples are stable for 14 days from the date of sample collection. If this sample is being collected for reflex to genotyping, do not submit in a Digene Cervical Sampler. The correct submission for reflex to genotyping is a SurePath or ThinPrep sample. Acceptable: Cervical biopsy: 2-5 mm2 in cross section placed immediately into Digene specimen transport medium and frozen at -20°C or SurePath vial if cytology is also requested, or SurePath CytoRich fraction if cytology is not requested.


Specimen Stability
Digene® specimen
Room temperature: 14 days
Refrigerated: 21 days
Frozen: 90 days
ThinPrep® PreservCyt®
Room temperature: 90 days
Refrigerated: 90 days
Frozen: Unacceptable
SurePath™ CytoRich® Fraction (pellet)
Room temperature: 14 days (from date of collection)
Refrigerated: 14 days
Frozen: Unacceptable
Cervical biopsy
Room temperature: 24 hours
Refrigerated: 24 hours
Frozen: Indefinitely


Methodology
Hybrid Capture II



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.