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A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
Heparin-Induced Platelet Antibody with Reflex to SRA, Unfractionated Heparin
Test Code15334
CPT Codes
86022
Includes
If Heparin-Induced Platelet Antibody is a weak positive or positive, then Serotonin Release Assay (SRA), Unfractionated Heparin will be performed at an additional charge (CPT code(s): 86022)
Preferred Specimen
1 mL serum collected in each of two separate red-top tubes (no gel)
Minimum Volume
0.5 mL (x2)
Instructions
Centrifuge red-top tube 15 minutes at approximately 1500 g as soon as possible after clotting
Transport Container
Transport tubes
Transport Temperature
Frozen
Specimen Stability
Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 6 months
Refrigerated: Unacceptable
Frozen: 6 months
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Lipemic • Icteric • Serum separator tube (SST)
Methodology
Immunoassay (IA)
Setup Schedule
Set up: Daily; Report available: 1-2 days
Reference Range
Heparin-Induced Platelet Ab | Negative |
Platelet O.D.
OD units | Results |
≤0.300 | Negative |
>0.300 to ≤0.500 | Weak Positive |
>0.500 | Positive |
Clinical Significance
Diagnosis of Heparin Induced Thrombocytopenia is based on clinical criteria but serological confirmation is often necessary. The ELISA assay measures the Platelet Factor 4/Heparin Complex to IgG. When the ELISA test is positive, testing by Serotonin Release Assay (a platelet activation assay) is more predictive of thrombocytopenia and thrombosis.