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HIV 1 RNA, Qualitative Real-Time PCR
Test Code16185
CPT Codes
87535
Preferred Specimen
1.6 mL serum
Minimum Volume
0.8 mL
Other Acceptable Specimens
Plasma collected in a PPT potassium EDTA (white-top) tube, or EDTA (lavender-top) tube
Instructions
Separate plasma or serum from the cells by centrifugation within 24 hours after collection. Transfer the specimen to a plastic screw-cap vial.
Separated plasma or serum can be sent in the PPT/SST but cannot be shipped frozen.
Separated plasma or serum can be sent in the PPT/SST but cannot be shipped frozen.
Transport Container
Plastic screw-cap vial
Transport Temperature
Frozen
Specimen Stability
Room temperature: 72 hours
Refrigerated: 5 days
Frozen: 42 days
Refrigerated: 5 days
Frozen: 42 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Whole blood • Unspun PPT • Frozen Plasma received in plasma preparation tube (in situ) • Frozen serum received in serum separator tube (in situ)
Methodology
Real-Time Polymerase Chain Reaction
Setup Schedule
Set up: Tues, Thurs, Sat; Report available: 1-5 days
Reference Range
Not detected
Clinical Significance
HIV-1 Qualitative is an in vitro nucleic acid amplification test for the qualitative detection of human immunodeficiency virus type 1 (HIV-1) RNA in human serum and plasma by PCR. The test is intended to be used as an aid in diagnosis of HIV-1 infection. Detection of HIV-1 nucleic acid is indicative of HIV-1 infection. The presence of HIV-1 nucleic acid in the plasma or serum of individuals without antibodies to HIV-1 is indicative of acute or primary HIV-1 infection. The HIV-1 Qualitative PCR may also be used as an additional test to confirm the presence of HIV-1 infection in an individual with specimens reactive for HIV-1 antibodies or antigens. The assay may also be used as an aid in the diagnosis of infection with HIV-1 in pediatric subjects and pregnant women. This assay is not intended to be used for monitoring patient status, or for screening donors of blood, plasma, or human cells, tissues, and cellular and tissue-based products (HCT/Ps) for HIV.