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Bordetella pertussis, PCR
MessageCulture is only available at request of Infectious Disease Epidemiology.
Test Code
CSBP
Alias/See Also
Bordetella pertussis (State)
CPT Codes
87801
Preferred Specimen
Nasopharyngeal Swab in leak-proof container
Instructions
Refer to the instruction sheet in the pertussis collection kit (linked below). Do not use wooden/calcium alginate swabs. Only synthetic-tipped (polyester, dacron, rayon) swabs should be used.
Send dry tube with swab. Refrigeration is not required for PCR only specimens.
Send dry tube with swab. Refrigeration is not required for PCR only specimens.
Specimen Stability
Room temperature: 24 hours
Refrigerated: 48 hours
Frozen: Unacceptable
Refrigerated: 48 hours
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Specimens collected with wooden shaft or calcium alginate swabs
Unlabeled, poorly labeled specimens, or leaking containers
Unlabeled, poorly labeled specimens, or leaking containers
Methodology
PCR
Setup Schedule
Daily
Report Available
3 Days
Reference Range
Bordetella spp DNA NOT Detected
Clinical Significance
Pertussis is an infectious respiratory disease caused by the Bordetella pertussis bacterium. Pertussis afflicts unvaccinated and previously vaccinated children, and adults in whom immunity has waned. Infants are at particular risk for severe disease and complications. Bordetella papapertussis may cause a similar illness, especially in children; however, the symptoms are less severe and of shorter duration than those associated with Bordetella pertussis.
Performing Laboratory
State of Maine Health and Environmental Testing Laboratory (HETL)
221 State Street SHS12
Augusta, ME 04333
(207) 441-7592
Additional Information
Maine State Lab Pertussis Submission Information Sheet
Instructions for Collecting Nasopharyngeal Specimens for Pertussis PCR