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Direct Antiglobulin Test (DAT)
MessageTest performed at York Hospital Laboratory.
Test Code
DAT
Alias/See Also
Direct Coombs
CPT Codes
86880
Preferred Specimen
12 mL whole blood collected in (2) EDTA (pink-top) tubes
Instructions
Specimen must be labeled with: (1) full patient name (2) date of birth (3) medical record number (inpatients only) (4) date and time of collection (5) identification of person who identified the patient (full legible signature and/or employee ID number is required. This labeling must occur in the presence of the patient.) The signature may be either printed or scripted but MUST be legible and written in indelible ink.
Transport Temperature
Refrigerated
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolyzed specimen
Methodology
Hemagglutination
Setup Schedule
Daily
Report Available
Next Day
Reference Range
Negative Critical Values Positive (Neonate)
Clinical Significance
Demonstrates in vivo coating of red blood cells with IgG or the complement component C3d in the following settings: Autoimmune hemolytic anemia, Hemolytic transfusion reactions, and Drug-induced hemolytic anemia.
