|
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
Cytomegalovirus DNA, Quantitative, Real-Time PCR
Test CodeCMVVL
Quest Code
10600
CPT Codes
87497
Preferred Specimen
1 mL whole blood or plasma collected in an EDTA (lavender- top) or ACD (yellow-top) tube or
1 mL plasma collected in EDTA PPT (white-top) tube or
1 mL serum or
1 mL CSF
1 mL plasma collected in EDTA PPT (white-top) tube or
1 mL serum or
1 mL CSF
Minimum Volume
0.5 mL
Instructions
Whole blood: Collect whole blood in sterile tubes containing EDTA or ACD as anticoagulant. Store refrigerated. Blood collected in tubes containing ACD anticoagulant will yield results approximately 15% lower when compared to EDTA tubes due to a dilution effect. Do not freeze whole blood.
Plasma: Collect blood in sterile tubes containing EDTA or ACD as anticoagulant or in plasma preparation tubes (PPTs). Store collected whole blood at room temperature and separate plasma from cells within 2 hours of collection. Transfer plasma to sterile plastic screw-cap tubes and store refrigerated or frozen. If blood is collected in a plasma preparation tube, centrifuge within 2 hours of collection and store refrigerated or frozen. It is not necessary to transfer the plasma from a plasma preparation tube to aliquot tubes. Blood collected in tubes containing ACD anticoagulant will yield results approximately 15% lower when compared to EDTA tubes due to a dilution effect.
Serum: Collect in a red top tube (no gel) or serum separator tube (SST).
CSF or amniotic fluid: Collect in a sterile container and store refrigerated or frozen.
Plasma: Collect blood in sterile tubes containing EDTA or ACD as anticoagulant or in plasma preparation tubes (PPTs). Store collected whole blood at room temperature and separate plasma from cells within 2 hours of collection. Transfer plasma to sterile plastic screw-cap tubes and store refrigerated or frozen. If blood is collected in a plasma preparation tube, centrifuge within 2 hours of collection and store refrigerated or frozen. It is not necessary to transfer the plasma from a plasma preparation tube to aliquot tubes. Blood collected in tubes containing ACD anticoagulant will yield results approximately 15% lower when compared to EDTA tubes due to a dilution effect.
Serum: Collect in a red top tube (no gel) or serum separator tube (SST).
CSF or amniotic fluid: Collect in a sterile container and store refrigerated or frozen.
Transport Container
Whole blood: EDTA (lavender-top) or ACD (yellow-top) tube
Plasma/serum: Transport tube
CSF: Sterile plastic screw-cap containter
Plasma/serum: Transport tube
CSF: Sterile plastic screw-cap containter
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Whole blood
Room temperature: 48 hours
Refrigerated: 8 days
Frozen: Unacceptable
Plasma/serum/CSF/amniotic fluid
Room temperature: 48 hours
Refrigerated: 8 days
Frozen: 30 days
Room temperature: 48 hours
Refrigerated: 8 days
Frozen: Unacceptable
Plasma/serum/CSF/amniotic fluid
Room temperature: 48 hours
Refrigerated: 8 days
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Heparinized specimens • Unspun PPT tube
Methodology
Real-Time Polymerase Chain Reaction (PCR)
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Day shift Mon, wed, fri
Report Available
3 days
Reference Range
CMV DNA, QN PCR | Not Detected | IU/mL |
CMV DNA, QN PCR | Not Detected | Log IU/mL |
Clinical Significance
In patients who are immunocompromised, CMV may cause disseminated, severe disease. CMV is also the most common cause of congenital viral infection in humans. Quantitative PCR methods may be useful in monitoring CMV replication in immunosuppressed patients or in determining the viral load of CMV in amniotic fluid. This is a quantitative molecular test, with a linear range of 200-2,000,000 IU/mL.
Performing Laboratory
Quest Diagnostics LLC |
200 Forest Street, 3rd Floor, Suite B |
Marlborough, MA 01752-3023 |