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Direct Antiglobulin Test (DAT) with Reflex to Anti C3 and Anti IgG
Test CodeDAGG
Quest Code
36668
Alias/See Also
Coombs, direct
CPT Codes
86880
Includes
If Direct Antiglobulin is positive, then Anti C3d and Anti IgG will be performed at an additional charge (CPT code(s): 86880 x2).
Preferred Specimen
5 mL whole blood collected in an EDTA (pink-top) tube
Minimum Volume
5 mL
Other Acceptable Specimens
Whole blood collected in: ACD (yellow-top) tube, EDTA (lavender-top) tube or Cord blood collected in a red top tube (no gel)
Instructions
If submitting with a CBC, HbA1c, or any other test requiring an EDTA (lavender-top) tube, please submit a separate tube for this test
Transport Container
EDTA (pink-top) tube
Transport Temperature
Room temperature
Specimen Stability
Room temperature: 24 hours
Refrigerated: 72 hours
Frozen: Unacceptable
Refrigerated: 72 hours
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Grossly lipemic • Serum separator tube (SST) • Clotted specimen• Grossly icteric
Methodology
Agglutination
Setup Schedule
Day shift Mon-fri
Report Available
2 days
Reference Range
Negative
Clinical Significance
The DAT (Direct Coomb's test) is positive if red cells have been coated, in vivo, with immunoglobulin, complement, or both. A positive result can occur in immune-mediated red cell destruction, autoimmune hemolytic anemia, a transfusion reaction or in patients receiving certain drugs.
Performing Laboratory
| Quest Diagnostics LLC |
| 200 Forest Street, 3rd Floor, Suite B |
| Marlborough, MA 01752-3023 |
