Hepatitis B Surface Antigen with Reflex Confirmation

Test Code

QHBSG

Quest Code

498

CPT Codes
87340

Includes

Positive samples will be confirmed based on the manufacturer's FDA approved recommendations at an additional charge (CPT code(s): 87341).

Preferred Specimen

2 mL serum

Minimum Volume

1 mL

Other Acceptable Specimens

Plasma collected in: EDTA (lavender-top) tube

Instructions

Dietary supplements containing biotin may interfere in assays and may skew analyte results to be either falsely high or falsely low. For patients receiving the recommended daily doses of biotin, draw samples at least 8 hours following the last biotin supplementation. For patients on mega-doses of biotin supplements, draw samples at least 72 hours following the last biotin supplementation.

Note: When only a single test, Hepatitis B Surface Antigen, is ordered to diagnose Hepatitis B in a pregnant woman, additional tests such as liver enzymes should be ordered to confirm the diagnosis.

Transport Container

Serum separator tube (SST)

Transport Temperature

Room temperature

Specimen Stability

Room temperature: 7 days
Refrigerated: 14 days
Frozen: 30 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Gross hemolysis • Grossly lipemic

Methodology
Immunoassay (IA)

Setup Schedule

Day shift Mon-fri

Report Available

2 days

Limitations

Results obtained from immunosuppressed patients should be interpreted with caution. Patients receiving mouse antibody therapy may produce false-negative results.

Reference Range

Non-Reactive

Clinical Significance

Surface antigen usually appears in the serum after an incubation period of 1 to 6 months following exposure to Hepatitis B virus and peaks shortly after onset of symptoms. It typically disappears within 1 to 3 months. Persistence of Hepatitis B Surface Antigen for greater than 6 months is a prognostic indicator of chronic Hepatitis B infection.

Performing Laboratory

Quest Diagnostics LLC

200 Forest Street, 3rd Floor, Suite B

Marlborough, MA 01752-3023

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.