IgG, IgA, Indirect Immunofluorescence

Test Code

QIFA

Quest Code

16690

CPT Codes
88346, 88350

Preferred Specimen

7.5 mL (x2) whole blood collected in red/black tiger-top (SST)

Minimum Volume

7.5 mL whole blood • 3 mL serum

Other Acceptable Specimens

6 mL serum collected in a red-top tube (no gel)

Instructions

Draw 7.5 mL whole blood in each of two tubes (10 mL tiger-top vacutainer or generic serum separator tube). No spin necessary. Submit a minimum of 7.5 mL of blood. If only one tube is submitted, it should be full. If two tubes are submitted, the volume of both tubes combined should add up to at least 7.5 mL of blood.

Transport Container

Serum separator tube (SST) - red/black tiger-top

Transport Temperature

Refrigerated (cold packs)

Specimen Stability

Room temperature: 48 hours
Refrigerated: 7 days
Frozen: Unacceptable

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Received frozen

Methodology
Immunoassay (IA)

Setup Schedule

Mon-fri

Report Available

3-4 days

Reference Range

See Laboratory Report

Clinical Significance

These studies are complementary diagnostic and prognostic tools for autoimmune blistering diseases, connective tissue disorders, and vasculitides. It is a semiquantitative technique whereby a double immunolabeling is performed to evaluate the presence and titer of circulating anti-epithelial cell surface, anti-basement membrane, anti-nuclear, and antineutrophil cytoplasmic antibodies.

Performing Laboratory
Dermpath Diagnostics South Florida
895 Southwest 30th Avenue
Suite 101
Pompano Beach, FL 33069-4887

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.