A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
Treponema pallidum DNA, Qualitative Real-Time PCR
Test Code16595
Preferred Specimen
0.7 mL CSF collected in a plastic sterile leak-proof container or
1 genital lesion swab collected in an Aptima® Transport Tube, M4 media, VCM medium (green-cap) tube or equivalent (UTM)
1 genital lesion swab collected in an Aptima® Transport Tube, M4 media, VCM medium (green-cap) tube or equivalent (UTM)
Minimum Volume
0.3 mL CSF
Instructions
Genital lesion swab: Swab any visible lesions. Withdraw the swab without touching the skin. Immediately place the swab into the transport tube so that the tip of the swab is visible below the tube label. Carefully break the swab shaft at the score line against the side of the tube and discard the top portion of the swab shaft. Tightly screw the cap onto the tube.
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
CSF
Room temperature: 7 days
Refrigerated: 14 days
Frozen: 30 days
Swab in Aptima® Transport tube
Room temperature: 21 days
Refrigerated: 14 days
Frozen: 30 days
Swab in M4, VCM (UTM)
Room temperature: Unacceptable
Refrigerated: 14 days
Frozen: 30 days
Room temperature: 7 days
Refrigerated: 14 days
Frozen: 30 days
Swab in Aptima® Transport tube
Room temperature: 21 days
Refrigerated: 14 days
Frozen: 30 days
Swab in M4, VCM (UTM)
Room temperature: Unacceptable
Refrigerated: 14 days
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Transport tubes with 2 swabs • Aptima® transport tubes with non-Aptima® swabs
Methodology
Real-Time Polymerase Chain Reaction
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Set up: Mon-Sat; Report available: 2-3 days
Reference Range
Not detected
Clinical Significance
Detection of Treponema pallidum DNA by Real-Time PCR is a useful tool for the rapid diagnosis of primary, secondary, and tertiary syphilis.
Performing Laboratory
Quest Diagnostics Nichols Institute-San Juan Capistrano, CA |
33608 Ortega Highway |
San Juan Capistrano, CA 92675-2042 |