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Cortisol Response to ACTH
MessageDeliver all samples of stimulation test together if collected at the clinic. Samples must be clearly labeled as Baseline, 30 minute, and 60 minute.
Test Code
ICORTS
Alias/See Also
ACTH Stimulation, Baseline 30 minute, Baseline 60 minute
CPT Codes
82533
Includes
Baseline, 30 minute, and 60 minute cortisols.
Preferred Specimen
1.0 ml of whole blood (0.5 mL of plasma/serum)
Minimum Volume
0.2 mL of whole blood (0.1 mL of plasma/serum)
Instructions
Provide dose and drug information.
Transport Container
Serum Separator preferred. Heparin plasma and EDTA plasma are acceptable.
Transport Temperature
Refrigerated
Specimen Stability
Room Temperature: 8 hours
Refrigerated: 5 days
Frozen: 4 weeks at - 20 C
Refrigerated: 5 days
Frozen: 4 weeks at - 20 C
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis or turbidity
Methodology
ENGU (Competitive Immunoassay)
Setup Schedule
Monday through Saturday
Report Available
Specimens received by noon will be reported by 4:30 p.m. Specimens received after noon will be reported by the next morning.
Clinical Significance
The cortisol response to infusion of synthetic ACTH is useful in diagnosing patients with adrenal insufficiency (Addison's disease).
