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Rapid Respiratory DFA Viral Screen with Reflex
MessageRapid Respiratory DFA VIral Screen with Reflex
Test Code
14860X
CPT Codes
87300
Preferred Specimen
2 mL nasopharyngeal lavage/wash or bronchial lavage/wash or tracheal lavage/wash or nasopharyngeal aspirate or nasopharyngeal swab or throat swabs on patients > 2 years old collected in a V-C-M medium (green-cap) tube or equivalent (UTM) or Multi Microbe Media (M4)
Instructions
Specimen Collection and Preparation:
Specimens for viral, Chlamydia, mycoplasmal or ureaplasmal investigation should be collected and handled following industry standard protocols. To maintain optimum viability, transport the specimen to the laboratory as soon as possible. Best recovery is obtained when the specimens are refrigerated at 2-8° C or kept on wet ice following collection and while in transit. If there will be a long delay before processing, specimens should be frozen at -70° C or colder and transported on dry ice. Storage at -20° C is less satisfactory than storage at 4° C or -70° C and can result in the loss of infectivity. For shipping and handling of specimens, follow state and federal regulations. Institutional guidelines should be followed to handle samples within the laboratory. All specimens should be processed as soon as they are received in the laboratory.
Specimen Collection:
Proper specimen collection from the patient is extremely critical for successful isolation and identification of infectious organisms. For specific guidance regarding specimen collection procedures follow industry standards for collecting infectious organisms. Specimens should be collected as soon as possible after clinical onset of disease. Highest viral titers are present during the acute illness.
For V-C-M medium vials:
1. Aseptically remove cap from vial.
2. Aseptically place sample into the vial with medium.
3. Replace cap on vial and close tightly.
4. Label with appropriate patient information.
5. Send to the laboratory for immediate analysis.
Specimens for viral, Chlamydia, mycoplasmal or ureaplasmal investigation should be collected and handled following industry standard protocols. To maintain optimum viability, transport the specimen to the laboratory as soon as possible. Best recovery is obtained when the specimens are refrigerated at 2-8° C or kept on wet ice following collection and while in transit. If there will be a long delay before processing, specimens should be frozen at -70° C or colder and transported on dry ice. Storage at -20° C is less satisfactory than storage at 4° C or -70° C and can result in the loss of infectivity. For shipping and handling of specimens, follow state and federal regulations. Institutional guidelines should be followed to handle samples within the laboratory. All specimens should be processed as soon as they are received in the laboratory.
Specimen Collection:
Proper specimen collection from the patient is extremely critical for successful isolation and identification of infectious organisms. For specific guidance regarding specimen collection procedures follow industry standards for collecting infectious organisms. Specimens should be collected as soon as possible after clinical onset of disease. Highest viral titers are present during the acute illness.
For V-C-M medium vials:
1. Aseptically remove cap from vial.
2. Aseptically place sample into the vial with medium.
3. Replace cap on vial and close tightly.
4. Label with appropriate patient information.
5. Send to the laboratory for immediate analysis.
Transport Container
See Preferred Specimen for specimen type being collected.
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Nasopharyngeal lavage/wash or bronchial lavage/wash or
tracheal lavage/wash or nasopharyngeal aspirate or
nasopharyngeal swab or throat swabs on patients > 2 years
old in V-C-M medium (green-cap) tube or equivalent (UTM) or Multi Microbe Media (M4)
Room temperature: Unacceptable
Refrigerated: 4 days
Frozen -20° C: Unacceptable
Frozen -70° C: 30 days
Nasopharyngeal lavage/wash or bronchial lavage/wash in sterile screw cap container
Room temperature: Unacceptable
Refrigerated: 72 hours
Frozen: Unacceptable
tracheal lavage/wash or nasopharyngeal aspirate or
nasopharyngeal swab or throat swabs on patients > 2 years
old in V-C-M medium (green-cap) tube or equivalent (UTM) or Multi Microbe Media (M4)
Room temperature: Unacceptable
Refrigerated: 4 days
Frozen -20° C: Unacceptable
Frozen -70° C: 30 days
Nasopharyngeal lavage/wash or bronchial lavage/wash in sterile screw cap container
Room temperature: Unacceptable
Refrigerated: 72 hours
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Throat swabs on patients <2 years old. • Formalin or other fixative samples. • Nucleic acid transport systems. • Non-respiratory sites • Whole blood • Serum • Plasma. • Dry swabs. • Frozen specimens.
Methodology
Direct Fluorescence Assay (DFA)
Setup Schedule
Set up: Daily a.m.; Report available: 1 day
Clinical Significance
Influenza viruses are markedly epidemic and known to cause pandemics. 'Influenza season' takes place during colder weather, generally regarded as October-May. Complications are a concern in the young, the elderly and persons with chronic cardio-pulmonary diseases. Superinfections with other viruses and bacteria may occur. Parainfluenza viruses cause croup and pneumonia in children under the age of 5 and is the second leading cause of lower respiratory illness. These cause upper respiratory illness in adults.
RSV causes mild infections in immunocompetent adults and bronchiolitis and pneumonia in infants, young children and immunocompromised/immunosuppressed adults. Simultaneous co-infections of parainfluenza 3 and RSV have been well documented.
Adenovirus may cause respiratory illness as well as hepatitis, meningitis, encephalitis, diarrhea and rash. The virus may be shed over long periods of time and be found in respiratory, eye, fecal or urine specimens.
RSV causes mild infections in immunocompetent adults and bronchiolitis and pneumonia in infants, young children and immunocompromised/immunosuppressed adults. Simultaneous co-infections of parainfluenza 3 and RSV have been well documented.
Adenovirus may cause respiratory illness as well as hepatitis, meningitis, encephalitis, diarrhea and rash. The virus may be shed over long periods of time and be found in respiratory, eye, fecal or urine specimens.
