HLA B27 Antigen

Test Code
HLAB27


CPT Codes
86812

Preferred Specimen
5 mL whole blood collected in sodium heparin (green-top) tube


Minimum Volume
3.0 mL whole blood collected in sodium heparin (green-top) tube


Other Acceptable Specimens
EDTA (lavender-top) tube,  ACD-A or ACD-B (yellow-top) tube


Transport Container
Sodium heparin (green top), EDTA (lavender-top) tube, ACD-A or ACD-B (yellow-top) tube


Transport Temperature
Room Temperature


Specimen Stability
Room temperature: 5 days
Refrigerated: 5 days
Frozen: Unacceptable


Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Received frozen


Methodology
Flow Cytometry

Setup Schedule
Tuesday through Sunday upon receipt at Quest Diagnostics


Report Available
Report available in 2 to 4 days of receipt at Quest Diagnostis


Clinical Significance
HLA-B27 is found in 90% of patients with ankylosing spondylitis and 80% in Reiter's disease. Ankylosing spondylitis affects 1 in 1000 caucasians. Ankylosing spondylitis is 10 times more common among individuals with HLA-B27 compared to individuals without this antigen.




The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.