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HLA B27 Antigen
Test CodeHLAB27
CPT Codes
86812
Preferred Specimen
5 mL whole blood collected in sodium heparin (green-top) tube
Minimum Volume
3.0 mL whole blood collected in sodium heparin (green-top) tube
Other Acceptable Specimens
EDTA (lavender-top) tube, ACD-A or ACD-B (yellow-top) tube
Transport Container
Sodium heparin (green top), EDTA (lavender-top) tube, ACD-A or ACD-B (yellow-top) tube
Transport Temperature
Room Temperature
Specimen Stability
Room temperature: 5 days
Refrigerated: 5 days
Frozen: Unacceptable
Refrigerated: 5 days
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Received frozen
Methodology
Flow Cytometry
Setup Schedule
Tuesday through Sunday upon receipt at Quest Diagnostics
Report Available
Report available in 2 to 4 days of receipt at Quest Diagnostis
Clinical Significance
HLA-B27 is found in 90% of patients with ankylosing spondylitis and 80% in Reiter's disease. Ankylosing spondylitis affects 1 in 1000 caucasians. Ankylosing spondylitis is 10 times more common among individuals with HLA-B27 compared to individuals without this antigen.