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Factor VIII Assay
Test CodeFAC8
Alias/See Also
Factor 8 Activity
CPT Codes
85240
Preferred Specimen
4.5 ml of whole blood preferred (tube must be filled to the line)
Minimum Volume
2.7 ml of whole blood preferred (tube must be filled to the line)
Instructions
Collect one full sodium citrate tube (blue top). Send to lab immediately. If the specimen needs to be transported, centrifuge the specimen, aliquot the specimen into a separate container, spin again, aliquot the specimen into a new container and freeze.
Transport Container
Sodium citrate (blue top)
Transport Temperature
Frozen
Specimen Stability
Specimen must be centrifuged within one hour of collection.
Room Temperature: 4 hours (centrifuged)
Refrigerated: Not acceptable
Frozen: 30 days
Room Temperature: 4 hours (centrifuged)
Refrigerated: Not acceptable
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Specimens which are not properly filled do not contain the proper anti-coagulant to blood ratio and are therefore rejected. Hemolyzed and clotted specimens are also unacceptable for testing.
Methodology
Clotting aPTT Based
Setup Schedule
Daily
Report Available
Turn around time: 24 hours for routine, 1.5 hours stat.
