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Factor V Leiden Screen with Reflex to Confirmation
MessageIf the screen is positive testing will reflex to Factor V Leiden Confirmatory, Mutation
Test Code
APC
Alias/See Also
Thrombosis, Thrombophilia, APC Resistance Profile
CPT Codes
85307
Preferred Specimen
(2) 4.5 ml whole blood sodium citrate tubes (blue tops) and (1) 4 mL EDTA (purple top)
Minimum Volume
(1) 4.5 ml whole blood sodium citrate tubes (blue top) and (1) 4 mL EDTA (lavendar top)
Instructions
Collect two full sodium citrate tube (blue top). Send to lab immediately. If the specimen needs to be transported, centrifuge the sodium citrate tubes, aliquot the specimen into a separate container, spin again, aliquot the specimen into a new container and freeze immediately. Send EDTA tubes whole blood room temperature or refrigerated.
Transport Container
Sodium citrate (blue top) and EDTA (lavender top)
Transport Temperature
Frozen
Specimen Stability
Sodium citrate tubes must be centrifuged within one hour of collection. Send EDTA whole blood room temperature or refrigerated.
Sodium citrate tubes:
Room Temperature: Not acceptable
Refrigerated: 4 hours (centrifuged)
Frozen: 30 days
Sodium citrate tubes:
Room Temperature: Not acceptable
Refrigerated: 4 hours (centrifuged)
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Specimens which are not properly filled do not contain the proper anti-coagulant to blood ratio and are therefore rejected. Hemolyzed and clotted specimens are also unacceptable for testing.
Methodology
Clotting
Setup Schedule
Set up Monday and Thursday
Report Available
Report in 1 to 5 days. Reflex testing will report within 3 to 4 days of receipt at Quest Diagnostics Nichols Institute.
