17-Hydroxypregnenolone

Test Code
8352X

CPT Codes
84143

Specimen Container
No additive (red-top)

Preferred Specimen
2 mL serum (0.4 mL minimum).

Transport Temperature
Refrigerated

Reject Criteria
Received room temperature
Serum in SST tube

Methodology
Extraction, Chromatography, Radioimmunoassay

Reference Range

Males and Females	20-450 ng/dL
Post-ACTH Stimulation	60 Minutes
Males & Premenopausal
Females (Follicular Phase)	290-910 ng/dL
Pediatric Reference Ranges for 17 Hydroxypregnenolone, Serum
Premature Infants
(31-35 weeks)	2409 ng/dL or less
Term Infants, 3 days old	830 ng/dL or less

ACTH Simulation	Baseline	60 Minutes

1-12 Months	14-830 ng/dL	395-3290 ng/dL
1-5 Years	10-100 ng/dL	45-740 ng/dL
6-12 Years	11-190 ng/dL	70-660 ng/dL

Tanner Stages II-III
Males	20-360 ng/dL	88-675 ng/dL
Females	58-450 ng/dL	250-800 ng/dL
Tanner Stages IV-V
Males	32-300 ng/dL	220-860 ng/dL
Females	53-540 ng/dL	500-1600 ng/dL

Pediatric data from J Clin Endocrinol Metab (1991) 73:674-686 and J Clin Endocrinol Metab (1989) 69:1133-1136

Clinical Use
17-Hydroxypregnenolone is useful in the diagnosis of 3-Beta-Hydroxylase enzyme deficiency, a rare cause of congenital adrenal hyperplasia, and 17-Hydroxylase (P450c17) enzyme deficiency.

Performed By
Quest Diagnostics Nichols Institute