17-Hydroxyprogesterone, LC/MS/MS

Test Code
17180X

CPT Codes
83498

Specimen Container
No additive (red-top)

Preferred Specimen
1 mL serum

Instructions
SST tubes are unacceptable. Draw blood in a no-additive (red-top) tube. Separate serum after clotting. Ship serum refrigerated or frozen. Do not submit glass tubes.

Transport Temperature
Frozen, stable 2 years

Reject Criteria
Received room temperature

Methodology
Liquid Chromatography Tandem Mass Spectrometry

Reference Range

Infants (1-5 days) Premature (31-35 weeks)*:	-< or = 360 ng/dL
Term (36-42 weeks)*:	-< or = 420ng/dL
1 - 12 months:	11 - 170 ng/dL*
1 - 4 years:	4 - 115 ng/dL*
5 - 9 years:	90 ng/dL or less
10 - 13 years:	169 ng/dL or less
14 - 17 years:	16 - 283 ng/dL

Tanner Stages
II-III: Males	12 - 130 ng/dL
II-III; Females	18 - 220 ng/mL
IV-V Males	51 - 190 ng/dL
IV-V Females	36 - 200 ng/mL

Adult Females

Follicular Phase:	< or = 185 ng/dL
Luteal Phase:	< or = 285 ng/dL
Postmenopausal Phase:	< or = 45 ng/dL
Pregnancy
First Trimester:	78 - 457 ng/dL
Second Trimester:	144 - 578 ng/dL

Males

18-30 years:	32-307 ng/dL
31-40 years:	42 -196 ng/dL
51 - 60 years:	37 - 129 ng/dL
>=60 years:	Not Established
*Includes data from J Clin Endocrinol Metab.1991; 73:674-686; J Clin Endocrinol Metab. 1989;69:1133-1136;and J Clin Endocrinol Metab. 1994;78:226-270

Clinical Use
17-hydroxyprogesterone is elevated in patients with congenital adrenal hyperplasia (CAH). CAH is a group of autosomal recessive diseases characterized by a deficiency of cortisol and an excess of ACTH concentration. 17-hydroxyprogesterone is also useful in monitoring cortisol replacement therapy and in evaluating infertility and adrenal and ovarian neoplasms.

Performed By
Quest Diagnostics Nichols Institute