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Factor II Prothrombin 331
Test CodeFAC2
Alias/See Also
FAC2
CPT Codes
85210
Preferred Specimen
1 mL platelet-poor plasma collected in a 3.2% sodium citrate (light blue-top) tube
Minimum Volume
1
Instructions
Draw light blue (sodium citrate and send platelet-poor plasma frozen with 30 min of drawing specimen
Transport Container
BLUE
Transport Temperature
F
Specimen Stability
Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 28 days
Refrigerated: Unacceptable
Frozen: 28 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis
Methodology
Photometric Clot Detection
Setup Schedule
Set up: Sun, Tues, Thurs, Fri; Report available: 1-4 days
Limitations
Expected impact by therapeutic levels (potential interference depends upon drug concentration): Warfarin: decreased, Factor V-no effect; Heparin (UFH or LMWH): no effect (reagent contains heparin neutralizer); Dabigatran or Argatroban (Thrombin Inhibitors): no effect to inhibitor pattern or falsely low; Rivaroxaban or Apixaban (Factor Xa Inhibitors): no effect to inhibitor pattern.
Reference Range
70-150 % normal
Clinical Significance
Factor II Activity, Clotting - The Factor II Activity clotting assay provides a quantitative level of Factor II (also known as Prothrombin) activity. Factor II/Prothrombin is essential for normal hemostasis and deficiencies are associated with increased bleeding risk. As a vitamin K- dependent clotting factor, decreased levels are observed with vitamin K antagonists (eg. warfarin therapy) and vitamin K deficiency. Deficiencies may also occur with hepatic dysfunction or as a result of inhibitor development. Antibodies to prothrombin can occur in patients with anti- phospholipid antibodies and can cause significant clinical bleeding; however, because these antibodies bind to a nonactive portion of the molecule, increased clearance occurs, so that a deficiency rather than an inhibitor is detected. Congenital Factor II deficiency is extremely rare. Anticoagulant interference: if not clinically contraindicated, avoid warfarin therapy for approximately 2 weeks before testing. Direct Xa or thrombin inhibitor therapy may cause falsely low levels (avoid for approximately 2-3 days before testing if not clinically contraindicated.
The Factor II Activity, Clotting assay IS NOT intended to identify the Prothrombin 20210G>A Gene Mutation, which is associated with the increased thrombotic risk.
The Factor II Activity, Clotting assay IS NOT intended to identify the Prothrombin 20210G>A Gene Mutation, which is associated with the increased thrombotic risk.
Performing Laboratory
Quest Diagnostics Nichols Institute |
14225 Newbrook Drive |
Chantilly, VA 20153 |