Alkaline Phosphatase, Bone Specific

Test Code
29498

CPT Codes
84075

Preferred Specimen
1 mL serum

Minimum Volume
0.3 mL

Other Acceptable Specimens

Serum separator tube

Instructions
Allow serum samples to clot completely before centrifugation

Transport Container
Plastic screw-cap vial

Transport Temperature
Room temperature

Specimen Stability
Room temperature: 7 days
Refrigerated: 7 days
Frozen: 60 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Gross lipemia • Non-serum samples • Grossly icteric

Methodology
Immunoenzymatic

Reference Range

Adult Male
18-29 Years	8.4-29.3 mcg/L
30-39 Years	7.7-21.3 mcg/L
40-49 Years	7.0-18.3 mcg/L
50-68 Years	7.6-14.9 mcg/L
Adult Female
18-29 Years	4.7-17.8 mcg/L
30-39 Years	5.3-19.5 mcg/L
40-49 Years	5.0-18.8 mcg/L
50-76 Years	5.6-29.0 mcg/L
Premenopausal
35-45 Years	5.0-18.2 mcg/L

Pediatric	Male (mcg/mL)	Female (mcg/mL)
2-24 months*	25.4-124.0	25.4-124.0
25 months-5 Years	Not established	Not established
6-9 Years	41.0-134.6	41.0-134.6
10-13 Years	43.8-177.4	24.2-154.2
14-17 Years	13.7-128.0	10.5-75.2

*Pediatric data from Int J Biol Markers 1996; 11:159-164.

Clinical Significance
The Bone-Specific Alkaline Phosphatase (BSAP) assay provides a general index of bone formation and a specific index of total osteoblast activity. BSAP and osteocalcin are the most effective markers of bone formation and are particularly useful for monitoring bone formation therapies and antiresorptive therapies.

Performed By
Quest Diagnostics Nichols Institute