HIV 1/2 EIA Antibody Screen with Reflexes

Test Code

CPT Codes
This is for non-New York patient testing only. For New York patient testing, refer to test code 17659X [12242] HIV Antibody, HIV-1/2, EIA with Reflexes (NY).

If the HIV Antibody Screen (HIV-1/2) is "Repeatedly Reactive", HIV Antibody, HIV-1, Western Blot will be performed at an additional charge (CPT code(s): 86689).
If the HIV Antibody, HIV-1, Western Blot is "Indeterminate" or "Negative", HIV-2 Antibody, EIA will be performed at additional charge (CPT code(s): 86702).
If the HIV-2 Antibody, EIA is "Repeatedly Reactive", HIV-2 Antibody, Immunoblot will be performed at an additional charge (CPT code(s): 86689).

Preferred Specimen
2 mL serum collected in a Serum Separator Tube (SST®)

Minimum Volume
1 mL

Other Acceptable Specimens
Serum collected in: Red-top tube (no gel)

Tube must be labeled with patient identifier and submitted only for HIV testing. HIV-1 Western Blot requires the original tube for testing. Tube should be spun after clotting and remain unopened.

Transport Temperature
Room temperature

Specimen Stability
Room temperature: 7 days
Refrigerated: 14 days
Frozen: 30 days

Immunoassay (IA)

HIV antibodies may not be detected in window between infection and detection of antibody.

Reference Range
HIV-1/2 Ab Screen, EIA Non-Reactive

A Non-reactive HIV-1/2 antibody result does not exclude HIV infection since the time frame for seroconversion is variable. If acute HIV infection is suspected, antibody retesting and nucleic acid amplification (HIV DNA/RNA) testing is recommended.

Clinical Significance
The combination HIV-1/HIV-2 includes detection of subtypes of HIV-1 not included in HIV-1, EIA alone. Repeatedly positive results are confirmed by the highly specific western blot.

Performed By
Quest Diagnostics Nichols Institute

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.