HIV 1/2 EIA Antibody Screen with Reflexes

Test Code
19728

CPT Codes
86703

Includes
If HIV Antibodies, HIV-1/2, EIA is Repeatedly Reactive, HIV-1/2 Antibody Differentiation will be performed at an additional charge (CPT code(s): 86701, 86702).

If HIV-1/2 Antibody Differentiation is Negative or Indeterminate, HIV-1 RNA, Qualitative, TMA will be performed at additional charge (CPT code(s): 87535).

Preferred Specimen
3 mL serum

Minimum Volume
2 mL

Other Acceptable Specimens
Plasma collected in an EDTA (lavender-top) tube

Instructions
Tube must be labeled with patient identifier and submitted only for HIV testing. HIV-1/2 Antibody Differentiation requires the original tube for testing. Tube should be spun after clotting and remain unopened.



Note to clients: Effective 4/6/2015, Quest Diagnostics will no longer offer the current 3rd Generation HIV diagnostic screening assay, test code 19728 HIV Antibodies, HIV-1/-2 EIA, with Reflexes. At that time, Quest Diagnostics will only offer test code 91431 HIV -1/2 Antigen and Antibodies, Fourth Generation, with Reflexes as recommended by the CDC. This HIV diagnostic screening assay tests for antibodies to HIV-1 and HIV-2 and HIV p24 antigen and provides greater sensitivity for detection of recent infection. Any orders for the 3rd Generation assay will automatically be referred to the 4th Generation assay.

Please note: This information has been disclosed to you from records whose confidentiality may be protected by state law. If your state requires such protection, then the state law prohibits you from making any further disclosure of the information without the specific written consent of the person to whom it pertains, or as otherwise permitted by law. A general authorization for the release of medical or other information is not sufficient for this purpose.

Transport Container
Plastic screw-cap vial or original collection tube used only for HIV Screen with patient's name or ID number

Transport Temperature
Room temperature

Specimen Stability
Room temperature: 72 hours

Refrigerated: 5 days

Frozen: 30 days

Methodology
Immunoassay (IA)

Limitations
HIV antibodies may not be detected in window between infection and detection of antibody.

Reference Range
HIV-1/2 Ab Screen, EIA Non-Reactive

A Non-reactive HIV-1/2 antibody result does not exclude HIV infection since the time frame for seroconversion is variable. If acute HIV infection is suspected, antibody retesting and nucleic acid amplification (HIV DNA/RNA) testing is recommended.

Clinical Significance
The combination HIV-1/HIV-2 includes detection of subtypes of HIV-1 not included in HIV-1, EIA alone. Repeatedly positive results are confirmed utilizing a different assay.

Performed By
Quest Diagnostics Nichols Institute



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.