HIV 1/2 EIA Antibody Screen with Reflexes

Test Code

CPT Codes

If HIV Antibodies, HIV-1/2, EIA is Repeatedly Reactive, HIV-1/2 Antibody Differentiation will be performed at an additional charge (CPT code(s): 86701, 86702).

If HIV-1/2 Antibody Differentiation is Negative or Indeterminate, HIV-1 RNA, Qualitative, TMA will be performed at additional charge (CPT code(s): 87535).

Preferred Specimen
3 mL serum

Minimum Volume
2 mL

Other Acceptable Specimens
Plasma collected in an EDTA (lavender-top) tube

Tube must be labeled with patient identifier and submitted only for HIV testing. HIV-1/2 Antibody Differentiation requires the original tube for testing. Tube should be spun after clotting and remain unopened.

Transport Container
Plastic screw-cap vial or original collection tube used only for HIV Screen with patient's name or ID number

Transport Temperature
Room temperature

Specimen Stability
Room temperature: 72 hours

Refrigerated: 5 days

Frozen: 30 days

Immunoassay (IA)

HIV antibodies may not be detected in window between infection and detection of antibody.

Reference Range
HIV-1/2 Ab Screen, EIA Non-Reactive

A Non-reactive HIV-1/2 antibody result does not exclude HIV infection since the time frame for seroconversion is variable. If acute HIV infection is suspected, antibody retesting and nucleic acid amplification (HIV DNA/RNA) testing is recommended.

Clinical Significance
The combination HIV-1/HIV-2 includes detection of subtypes of HIV-1 not included in HIV-1, EIA alone. Repeatedly positive results are confirmed utilizing a different assay.

Performed By
Quest Diagnostics Nichols Institute

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.