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Yellow Hornet (i5) IgE
Test CodeYLHN
CPT Codes
86003
Preferred Specimen
0.3 mL Serum (Min 0.15mL)
Transport Container
Serum Separator Tube
Transport Temperature
Room Temperature
Methodology
Immunoassay
Reference Range
Accompanies report
Clinical Significance
This procedure is based on a fluorescent enzyme immunoassay (FEIA) technique. The allergen of interest, covalently coupled to an ImmunoCap, reacts with the specific IgE in the patients serum. After washing away nonspecific IgE, enzyme-labelled antibodies against IgE are added to form a complex. After incubation, unbound
enzyme anti-IgE is washed away and the bound complex is then incubated with a developing agent. After the reaction is stopped, the fluorescence of the eluate is measured. The higher the fluorescence value, the more specific IgE is present in the specimen. To classify test results, fluorescence for patient samples is compared directly with fluorescence for a reference serum run in parallel. Results are reported in both kU/L and Conventional Class score.
enzyme anti-IgE is washed away and the bound complex is then incubated with a developing agent. After the reaction is stopped, the fluorescence of the eluate is measured. The higher the fluorescence value, the more specific IgE is present in the specimen. To classify test results, fluorescence for patient samples is compared directly with fluorescence for a reference serum run in parallel. Results are reported in both kU/L and Conventional Class score.
